Genital Mucosal Immunity in Adolescent Women

青春期女性的生殖器粘膜免疫

基本信息

批准号：
8786044
负责人：
REBECCA Pellett MADAN
金额：
$ 8.91万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-01-15 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8786044
关键词：
21 year old AIDS prevention Address Adolescence Adolescent Adult Affect Age Age Distribution Antiviral Agents Bacteria Biological Biological Assay Biological Markers Biological Response Modifiers CD4 Positive T Lymphocytes Cell Count Cell physiology Cervical Data Defensins Development Plans Disease Ecosystem Environment Epidemiologic Studies Evaluation Evolution Exhibits Female Female Adolescents Foundations Future Genital system Goals HIV HIV Infections HIV-infected adolescents Health High Risk Woman Host Defense Human Papillomavirus Hydrogen Peroxide Immune Immune response Immunoglobulins Immunologics In Vitro Infection Infection prevention Intervention Knowledge Laboratories Lactobacillus Lead Liquid substance Mediator of activation protein Menarche Menstruation Mentorship Mucosal Immunity Mucous Membrane Muramidase Mus Natural Immunity Ovulation Peptides Plasma Population Prevention Protein Array Recurrence Research Methodology Research Personnel Risk Safety Sampling Sex Behavior Sexually Transmitted Diseases Simplexvirus Swab Techniques Testing Training Translational Research Vaccines Vagina Viral Load result Woman antimicrobial antiretroviral therapy base career development cellulose sulfate cervicovaginal chemokine cohort cytokine experience genital secretion high risk hypothalamic pituitary ovarian axis meetings microbicide mucosal vaccine novel pathogen pathogenic bacteria prevent reproductive hormone response restoration sexual debut therapy development transmission process trend vaginal microbicide young woman

项目摘要

DESCRIPTION (provided by applicant): Adolescent and young women are at high risk for HIV and STI acquisition. Developing novel, safe, preventative options for this population, such as vaginal microbicides, requires a thorough understanding of mucosal immunity in the female genital tract. Prior studies have evaluated the genital mucosal immune environment in adult women and indicate that protective factors in cervicovaginal fluid exhibit intrinsic activity against HIV, herpes simplex virus, and multiple types of pathogenic bacteria. However these data are lacking in young, adolescent women, and it remains unknown how innate immunity in the genital tract develops during adolescence. Moreover little is known of the functional components of genital mucosal immunity in young women infected with HIV, who may use microbicides with no knowledge of their infection or who may wish to use a microbicide to prevent infection with another STI or to prevent HIV transmission to their partner. This application addresses these knowledge gaps through the following specific aims: [(1) evaluate the differences in genital mucosal immunity in HIV uninfected females ages 13-21 years who are either within three years of menarche or greater than three years from menarche by comparing endogenous antimicrobial activity in genital secretions between the two groups and correlating this activity with specific immune mediators and vaginal microbiota populations; (2) evaluate the differences in genital mucosal immunity between a cohort of HIV infected young women ages 13-21 years and a cohort of healthy, uninfected women of similar age distribution by comparing endogenous antimicrobial activity in genital secretions and correlating this activity with specific immune mediators and vaginal microbiota populations.] The results of these studies will have significant implications for future studies of HIV and STI prevention and acquisition in young women. My objective is to become an independent investigator with expertise in STI acquisition and prevention in adolescent women. I have proposed a career development plan that incorporates mentorship in translational research methods, specialized training in novel laboratory techniques, individualized coursework, and participation in local and national meetings.

描述（由申请人提供）：青少年和年轻女性感染艾滋病毒和性传播感染的风险很高。为这一人群开发新颖、安全的预防方案，例如阴道杀微生物剂，需要彻底了解女性生殖道的粘膜免疫。先前的研究评估了成年女性的生殖器粘膜免疫环境，并表明宫颈阴道液中的保护因子表现出针对艾滋病毒、单纯疱疹病毒和多种病原菌的内在活性。然而，年轻的青春期女性缺乏这些数据，而且青春期生殖道的先天免疫是如何发展的仍不清楚。此外，人们对感染艾滋病毒的年轻女性生殖器粘膜免疫的功能成分知之甚少，她们可能在不了解自己感染的情况下使用杀菌剂，或者可能希望使用杀菌剂来预防感染另一种性传播感染或防止艾滋病毒传播给她们。伙伴。本申请通过以下具体目标解决了这些知识差距：[(1) 通过比较内源性免疫，评估未感染 HIV 的 13-21 岁女性（月经初潮三年内或月经初潮三年以上）的生殖器粘膜免疫差异。两组之间生殖器分泌物的抗菌活性，并将该活性与特定的免疫介质和阴道微生物群相关联； (2) 通过比较生殖器分泌物中的内源性抗菌活性并将该活性与特异性免疫相关联，评估一组 13-21 岁感染 HIV 的年轻女性和一组年龄分布相似的健康、未感染女性的生殖器粘膜免疫差异。介质和阴道微生物群。]这些研究的结果将对年轻女性艾滋病毒和性传播感染预防和感染的未来研究产生重大影响。我的目标是成为一名独立调查员，拥有青春期女性性传播感染和预防方面的专业知识。我提出了一个职业发展计划，其中包括转化研究方法的指导、新颖实验室技术的专门培训、个性化课程以及参加地方和国家会议。