Comprehensive Lab Animal Monitor System (CLAMS) for the Study of Mouse Metabolism

用于小鼠代谢研究的综合实验动物监测系统 (CLAMS)

• 批准号: 8826468
• 负责人: ALEXANDER BANKS
• 金额: $ 41.92万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-10 至 2016-02-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826468
• 关键词: Animals; Body Temperature; Carbon Dioxide; Conscious; Diabetes Mellitus; Energy Intake; Energy Metabolism; Equilibrium; Exercise Tolerance; Funding; Heating; Indirect Calorimetry; Institution; Liver diseases; Measurement; Measures; Metabolic; Metabolic Diseases; Metabolism; Monitor; Motor Activity; Mus; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Oxygen Consumption; Production; Productivity; Protocols documentation; Request for Applications; Research; Research Personnel; Running; System; Technology; Temperature; Thermogenesis; Time; interest; mouse model; non-alcoholic fatty liver; obesity treatment; research study; respiratory

DESCRIPTION (provided by applicant): This application requests funds to upgrade and expand a Comprehensive Lab Animal Monitoring System (CLAMS) with environmental controls, which will markedly accelerate research and discovery, particularly in common metabolic disorders that include obesity, type 2 diabetes and non-alcoholic fatty liver disease. A CLAMS is utilized for indirect calorimetry of unrestrained, conscious mice, yielding quantitative measurements of oxygen consumption (VO2), carbon dioxide production (VCO2), respiratory exchange ratio - an indicator of substrate utilization - total energy expenditure, and body temperature. It also provides measures of basal locomotor activity and exercise tolerance, as well as caloric intake. Our existing CLAMS is specialized, allowing for the precise control of ambient temperature ranging from thermoneutrality (30�C) to cold (4�C). This feature is essential for the systematic quantification of thermogenesis, the capacity to generate body heat from stored energy. Mechanisms that control thermogenesis hold great promise as targets for the treatment of obesity and diabetes, but are poorly understood. The use of a CLAMS in our initial studies has revealed profound changes in energy substrate utilization and induction of thermogenesis in multiple mouse models. As a result, there is keen interest among investigators at our institution to expand the use of CLAMS in their metabolic research. Our existing CLAMS facility allows for study of only 6 mice at a time (3 control, 3 experimental). Because each experiment typically lasts for 1-2 weeks, and requires duplicate runs to increase the number of mice monitored, this has created long waiting times and difficulty with the planning and execution of protocols. The proposed expansion will allow us to monitor up to 24 mice per experiment. This capacity will provide an appropriate balance between the availability of the CLAMS and the demand for its use. Because CLAMS is an absolutely essential technology for the understanding of mouse metabolism, the proposed expansion will fulfill a critical need and increase research productivity.

描述（由应用程序提供）：此应用程序要求资金升级和扩展具有环境控制的全面的实验室动物监测系统（CLAM），这将显着加速研究和发现，特别是在常见代谢性疾病中，包括肥胖，2型糖尿病和非酒精性脂肪肝疾病。蛤用于间接量热法，对氧气消耗（VO2），二氧化碳的产生（VCO2），呼吸道交换比 - 底物利用率 - 总能量消耗和身体温度的指标进行定量测量。它还提供了基本的运动活性和运动耐受性以及热量摄入量的测量。我们现有的蛤是专门的，可以精确控制环境温度，从热结（30.C）到冷（4.c）。特征对于系统量化的热生成是必不可少的，即从存储能量产生人体热量的能力。控制生热的机制具有巨大的希望，作为治疗肥胖和糖尿病的靶标，但知之甚少。在我们的初步研究中，蛤的使用揭示了能量底物利用和在多种小鼠模型中热生成的诱导的深刻变化。结果，我们机构的研究人员对扩大蛤的使用在其代谢研究中的使用浓厚。我们现有的蛤设备一次只能研究6只小鼠（3个对照，3个实验）。由于每个实验通常持续1-2周，并且需要重复运行以增加监测的小鼠数量，因此这在计划和执行方案的计划和执行方面产生了较长的等待时间和困难。提出的扩展将使我们每个实验最多监视24只小鼠。这种能力将在蛤的可用性和对其使用的需求之间提供适当的平衡。由于蛤是理解小鼠代谢的绝对必要技术，因此提出的扩展将满足批判性需求并提高研究生产力。