Cellular and Molecular Biology

细胞和分子生物学

基本信息

批准号：
8828804
负责人：
John D. Houle
金额：
$ 3.45万
依托单位：
DREXEL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-15 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8828804
关键词：
Anatomy Biological Cell Line Cell Maintenance Cell Transplantation Cells Cellular biology Cloning Vectors Complementary DNA Core Facility Culture Media Data Analyses Databases Doctor of Philosophy Enzyme-Linked Immunosorbent Assay Enzymes Equipment Experimental Designs Fibroblasts GRP gene Genetic Growth Individual Injury Instruction Label Mediating Modeling Molecular Molecular Analysis Molecular Biology Molecular and Cellular Biology Neurobiology Neuroepithelial Cells Neurons Plasmids Population Procedures Quality Control Reagent Records Research Personnel Serum Site Spinal cord injury Structure of thyroid parafollicular cell Students System Technical Expertise Testing Therapeutic Time Tissues Training Transfection Transplantation Variant Viral Virus Western Blotting adeno-associated viral vector cellular transduction computerized cost effective genetically modified cells immortalized cell improved nerve stem cell programs recombinant virus repaired repository research study therapeutic gene tissue culture treatment strategy virology

项目摘要

CORE C: Cell and Molecular Biology - Ying Jin, Ph.D. - PI This Core has existed for 10 years within the Department of Neurobiology and Anatomy to facilitate the molecular analysis of cells and tissues in the SCI site and within transplants. Most importantly, the core prepares cells for grafting experiments in the individual projects. All projects will use the Cell and Molecular Core. The facility isolates, expands and stores primary fibroblasts, neural stem cells and cell lines as required. We genetically modify cells by viral transduction or transfection and provide quality control for all cells and reagents used in the core. The staff of the Cell and Molecular Core provides assistance for experimental design and data analysis. The core has developed a computerized ordering system in which individual investigators & staff request control, labeled or genetically modified cells for transplantation. Our records show that over the past 5 years the core has provided over 3 x 1 0 ^ cells, including unmodified and modified fibroblasts, neuronal and glial restricted precursors (NRP and GRP), neuroepithelial cells (NEP) and cells from immortalized cell lines. The core maintains viruses and transduced cell stocks in long-term storage and serves as a database and repository for cDNA clones, vectors, viruses and cells. Efficient and cost-effective ordering of tissue culture and molecular biology supplies as well as large scale testing of new sera and improved growth media, enzymes, kits or equipment needed for molecular analysis (thermocyclers, Q-PCR) are key core functions. We are also continuously developing new improved plasmids and viruses and have recently added and adeno-associated virus vector (AAV) as an option to our program. This will allow us to specifically transfect graft neural stem cells with growth promoting and therapeutic factors to be delivered at or near the site of injury.

Core C：细胞和分子生物学-Ying Jin，Ph.D。 - pi 该核心已经在神经生物学和解剖学系中存在了10年，以促进 SCI部位和移植中细胞和组织的分子分析。最重要的是，核心为单个项目中的嫁接实验准备细胞。所有项目都将使用细胞和分子核。设施分离株，扩展和存储原发性成纤维细胞，神经干细胞和细胞系，作为必需的。我们通过病毒转导或转染对细胞进行基因修饰，并为所有人提供质量控制芯中使用的细胞和试剂。细胞的工作人员和分子核心为实验设计和数据分析。核心已经开发了一个计算机化的订购系统个人研究人员和员工要求控制，标记或转基因细胞进行移植。我们的记录显示，在过去的5年中修饰的成纤维细胞，神经元和神经胶质限制前体（NRP和GRP），神经上皮细胞（NEP）以及永生的细胞系中的细胞。核心长期维持病毒并转导细胞库存存储并用作cDNA克隆，载体，病毒和细胞的数据库和存储库。高效和组织培养和分子生物学供应的成本效益有效的订购以及新的大规模测试血清和改进的生长培养基，酶，套件或分子分析所需的设备（热循环器，， Q-PCR）是关键核心功能。我们还不断开发新的改进的质粒和病毒并最近添加并与腺相关病毒载体（AAV）作为我们计划的选项。这会允许我们专门转染具有生长促进和治疗因素的移植神经干细胞在受伤部位或附近交付。