Bioanalytical CoreBioanalytical Core

生物分析核心Bioanalytical Core

• 批准号: 8899511
• 负责人: STEPHEN BARNES
• 金额: $ 23.12万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8899511
• 关键词: Acute Renal Failure with Renal Papillary Necrosis; Animal Model; Biochemistry; Bioenergetics; Biological Assay; Biological Markers; Cells; Cessation of life; Chronic Kidney Failure; Citric Acid; Citric Acid Cycle; Clinical; Communities; Consultations; Creatinine; Diagnosis; Disease; EGF gene; Educational workshop; Experimental Designs; F2-Isoprostanes; FABP1 gene; Funding; Glutathione Disulfide; Grant; Hospitalization; Human Resources; Instruction; Interferons; Interleukin-10; Interleukin-18; Interleukin-6; Iothalamate; Isoprostanes; Kidney; Lead; Leadership; Learning; Lipid Peroxidation; Mass Spectrum Analysis; Metabolism; Methods; Mitochondria; National Institute of General Medical Sciences; Osteocalcin; Oxidative Stress; Peer Review; Performance; Plasma; Post-Translational Protein Processing; Principal Investigator; Protocols documentation; Publications; Recovery; Research; Research Methodology; Research Personnel; Research Support; Resources; Sampling; Services; TNF gene; Techniques; Technology; Time; Tissue Inhibitor of Metalloproteinase-1; Tissues; Training; Training and Education; Transferase; UMOD gene; United States National Institutes of Health; Uric Acid; base; cost; experience; extracellular; investigator training; member; metabolomics; osteopontin; post gamma-globulins; programs; small molecule; urinary

PROJECT SUMMARY (See instructions): The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. Core C has been successful in supporting the kidney research community. Since the inception of Core C, more than 8,500 assays have been performed for 48 principal investigators involving 56 projects. Of the 48 investigators, 44 were non-core personnel. The number of investigators using Core C each year is increasing, as is the annual publication rate. Core C has also supported the research efforts of 7 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 23 peer-reviewed publications. The Core will participate in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature from 2013-2017 will be an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics). Finally, the Bioanalytical Core, in concert with the O'Brien Center leadership, will identify new bioanalytical needs of investigators and implement them for the Center.

项目摘要（请参阅说明）：通过在UAB和UCSD和扩展研究基础上为O'Brien Center社区提供生物分析核心（Core C），通过提供最先进的生物能学，氧化应激分析和急性肾脏损伤（AKI）研究的代谢物分析支持。 Core C提供了一种全面的资源，其中包括针对氧化应激和细胞生物能学的生物分析分析的优化方案和技术，AKI的生物标志物，翻译后修饰以及小分子生物化学的变化。该服务涉及咨询，实验设计培训，适合分析和测定性能的样品的恢复。提供的新服务包括确定线粒体生物能学的独特技术，基于LC-MAS光谱法的肌酐，F2-异丙烷和柠檬酸Cyle Cyle中间体的测定法。正在开发最先进的纳米MS方法，以在临床样本和AKI动物模型中同时执行靶向和未靶向的代谢组学和外泌体分析。 Core C已成功支持肾脏研究社区。自Core C成立以来，已经为48位涉及56个项目的主要研究人员进行了8500多次测定。在48位调查人员中，有44名是非核心人员。每年使用Core C的调查人员数量正在增加，年度发布率也是如此。 Core C还支持7个飞行员和可行性赠款获奖者的研究工作。这些合并的努力目前已在23个同行评审的出版物中得到认可。核心将参加提供动手经验和科学交流的调查人员的教育和培训。 2013 - 2017年的重复性功能将是一年一度的为期4天的代谢组学培训（由NIGMS资助，作为NIH共同基金计划的代谢组学领域）。最后，与奥布莱恩中心领导层协同的生物分析核心将确定调查人员的新生物分析需求，并为中心实施它们。