WU-MDACC Inter-Institutional Molecular Imaging Center

WU-MDACC 跨机构分子影像中心

基本信息

批准号：
8820886
负责人：
Samuel Achilefu
金额：
$ 142.89万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-05-31 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8820886
关键词：
Academic Medical Centers Address Alleles Allogenic Ara-C Basic Science Biochemical Reaction Biological Assay Biology Bioluminescence Bone Marrow Transplantation Cancer Center Cell Cycle Checkpoint Cells Chemistry Clinic Clinical Clinical Research Clinical Trials Collaborations Diagnostic Discipline Donor Lymphocyte Infusion Endothelial Cells Engineering Epigenetic Process Event Explosion Foundations Funding Gene Expression Genes Goals HIV Human Image Inflammation Interdisciplinary Study Investigation Knock-in Mouse Lead Libraries Life Luciferases Lymphocyte Malignant Neoplasms Medicine Metabolic stress Molecular Monitor Mus Neoplasms in Vascular Tissue Neoplastic Cell Transformation Organism Pathologic Processes Patients Pilot Projects Positron-Emission Tomography Postdoctoral Fellow Process Regulatory Pathway Relapse Reporter Research Research Personnel Research Project Grants Resources Robotics Role Series Signal Transduction Signaling Protein Simplexvirus Small Interfering RNA Starvation Stem cell transplant Students T-Lymphocyte Taxes Techniques Therapeutic Tissues Training Programs Transcriptional Regulation Transgenic Organisms Translating Translational Research Tumorigenicity Universities University of Texas M D Anderson Cancer Center Viral Washington Whole Organism cancer therapy chemical genetics chemotherapy clinical application conditioning experience fundamental research gene therapy clinical trial genetically modified cells graft vs host disease high throughput screening imaging platform in vivo in vivo Cellular and Molecular Imaging Centers innovation interest molecular imaging multidisciplinary mutant new therapeutic target notch protein novel novel strategies prognostic programs research study response trafficking tumor tumorigenesis virology

项目摘要

DESCRIPTION (provided by applicant): Ground-breaking molecular imaging studies have visualized gene expression, biochemical reactions, signal transduction and regulatory pathways in living cells and whole organisms in vivo. The Washington University Molecular Imaging Center directs major efforts into fundamental, translational and clinical research under the theme of imaging signal transduction and therapeutics in cancer using novel techniques and probes in combination with genetically-encoded reporters to pinpoint molecular events within target cells. The Washington University Molecular Imaging Center activities have spurred an explosion of collaborative, interdisciplinary research projects that have formed the foundation for exciting new directions in molecular imaging. A dedicated team of investigators have demonstrated an exceptional record of collaboration outside their own disciplines leading to an escalating interest in applying novel genetically- encoded reporter strategies that allow the molecular characterization of cancer in vivo for basic research as well as diagnostic and prognostic clinical applications. In particular, this has lead to a major theme to study regulated signal transduction and protein processing in living organisms. The principal research projects address imaging of Notch signaling in cancer, investigate metabolic stress and checkpoint controls as targets for anticancer therapy, investigate the interaction of inflammation and viral-induced tumorigenicity in doubly transgenic reporter mice, and monitor trafficking of genetically-modified cells with PET during epigenetic modulation of donor lymphocytes in a clinical study of patients undergoing bone marrow transplant. The program also includes three molecular imaging scientific resources: the molecular imaging reporter core, chemistry core and high throughput screening robotics core, founded on molecular imaging platforms for readout of a wide range of bio-assays, including siRNA library screens, and chemical genetics. Our Molecular Imaging Center also provides a pilot project program for new investigators and a multidisciplinary training program for students, post-docs, and fellows.

描述（由申请人提供）：突破性的分子成像研究使活细胞和整个生物体体内的基因表达、生化反应、信号转导和调控途径可视化。华盛顿大学分子成像中心以癌症成像信号转导和治疗为主题，主要致力于基础、转化和临床研究，利用新技术和探针结合基因编码报告基因来查明靶细胞内的分子事件。华盛顿大学分子成像中心的活动刺激了跨学科合作研究项目的激增，这些项目为分子成像领域令人兴奋的新方向奠定了基础。一个专门的研究团队在各自学科之外展示了卓越的合作记录，导致人们对应用新型基因编码报告策略的兴趣不断增加，这些策略允许对体内癌症进行分子表征，以用于基础研究以及诊断和预后临床应用。特别是，这引发了研究生物体中调节信号转导和蛋白质加工的主要主题。主要研究项目涉及癌症中Notch信号的成像，研究代谢应激和检查点控制作为抗癌治疗的目标，研究双转基因报告小鼠中炎症和病毒诱导的致瘤性的相互作用，并利用PET监测转基因细胞的运输在接受骨髓移植的患者的临床研究中，对供体淋巴细胞进行表观遗传调节。该计划还包括三个分子成像科学资源：分子成像报告核心、化学核心和高通量筛选机器人核心，这些资源建立在分子成像平台上，用于读取各种生物测定，包括 siRNA 库筛选和化学遗传学。我们的分子成像中心还为新研究人员提供试点项目计划，并为学生、博士后和研究员提供多学科培训计划。