Tsetse Fecundity Reduction for Trypanosomiasis Control

减少采采蝇繁殖力以控制锥虫病

基本信息

批准号：
8479307
负责人：
Serap AKSOY
金额：
$ 38.12万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8479307
关键词：
Adipose tissue Adult Africa South of the Sahara African African Trypanosomiasis Animals Antibiotics Bacteria Biological Birth Bite Blood Cessation of life Chemicals Communities Computer Simulation Deposition Development Diet Disease Vectors Elements Fat Body Female Fertility Genes Genomics Gland Goals Growth Health Hemolymph Human Indium Infection Insect Vectors Insecta Insecticides Investigation Knowledge Lactation Larva Lipid Mobilization Lipids Livestock Longevity Measures Mediating Metabolism Methods Milk Milk Proteins Molecular Molecular Target Mothers Movement Nature Nucleic Acid Regulatory Sequences Nutrient Oviparity Parasites Pattern Peptide Hormones Receptors Pharmaceutical Preparations Population Pregnancy Prevention Prevention strategy Promoter Regions Proteins Regulation Reproduction Reproductive Biology Reproductive Physiology Reproductive Process Reproductive system Resources Role Route Source Staging Sterility Supplementation System Time Tissues Trypanosoma Trypanosomiasis Tsetse Flies Vaccines Weight Wigglesworthia adipokinetic hormone dietary restriction dietary supplements disease transmission disorder control fitness fly health economics insect disease lipophorin lipophorin receptor novel nutrition offspring protein function receptor reproductive transcription factor transmission process vector vector control

项目摘要

DESCRIPTION (provided by applicant): The focus of this proposal is to identify molecular targets of tsetse fly reproductive physiology for the development of novel biological control strategies for the prevention of disease transmission. Tsetse are the vectors of trypanosomiasis in both humans and animals in sub-saharan Africa creating major health and economic problems. There are no vaccines to control disease in the mammalian host and only a single and highly toxic drug is available for treatment of late stage infections. Vector control methods, such as trapping and insecticide spraying, are effective in reducing fly challenge. Vector reduction methods are especially effective due to the low fly population numbers resulting from tsetse's slow reproductive rate. Tsetse has an unusual reproductive biology as they only develop one offspring at a time and give birth to a fully developed larva. Reproduction in tsetse is also unique in that it requires the presence of obligate mutualist symbiotic bacteria. Nutrients for the developing intrauterine larva are supplied entirely by the mother. Nutrients are transferred from the mother to offspring by milk secretions generated in a specialized accessory gland called the milk gland. Disruption of the flow of nutrients into the developing larva, or the elimination of symbiotic bacteria both result in loss of host fecundity. The application has three goals. The first goal focuses on the transfer of lipids from the fat body tissue to the milk gland and its regulation. It will identify and characterize the conserved peptide hormone/receptor system (adipokinetic hormone) responsible for regulating lipid mobilization in insects as well as the system responsible for the physical movement of lipids through the hemolymph (lipophorin/lipophorin receptor). The second goal focuses on the protein components of the milk secretion. It will identify novel milk proteins in silico and characterize the promoter and regulatory regions to understand the transcription factors responsible for milk gland/pregnancy specific expression patterns. The third goal focuses on the indispensable role of symbiotic bacteria in host reproductive processes. It will characterize the factors symbionts provide to facilitate host fecundity, analyze the expression patterns of host reproduction associated genes in symbiont-cured sterile flies. Collectively, the proposed studies will identify key elements required for tsetse reproductive processes. These elements stand to provide targets for tsetse specific vector control strategies for use in the prevention of trypanosomiasis.

描述（由申请人提供）：本提案的重点是确定采采蝇生殖生理学的分子靶标，以开发预防疾病传播的新型生物控制策略。采采蝇是撒哈拉以南非洲地区人类和动物锥虫病的传播媒介，造成严重的健康和经济问题。没有疫苗可以控制哺乳动物宿主的疾病，并且只有一种高毒性药物可用于治疗晚期感染。媒介控制方法，如诱捕和喷洒杀虫剂，可有效减少苍蝇挑战。由于采采蝇繁殖速度缓慢，苍蝇种群数量较少，因此减少媒介的方法尤其有效。采采蝇具有不寻常的生殖生物学，因为它们一次只发育一个后代，并产下发育完全的幼虫。采采蝇的繁殖也很独特，因为它需要专性共生共生细菌的存在。宫内幼虫发育的营养完全由母亲提供。营养物质通过称为乳腺的特殊辅助腺体产生的乳汁分泌物从母亲转移到后代。营养物质流入发育中的幼虫的中断或共生细菌的消除都会导致宿主繁殖力的丧失。该应用程序有三个目标。第一个目标侧重于脂质从脂肪体组织到乳腺的转移及其调节。它将鉴定和表征负责调节昆虫脂质动员的保守肽激素/受体系统（脂肪运动激素）以及负责脂质通过血淋巴物理运动的系统（脂蛋白/脂蛋白受体）。第二个目标侧重于乳汁分泌的蛋白质成分。它将在计算机中识别新型乳蛋白，并表征启动子和调节区域，以了解负责乳腺/妊娠特异性表达模式的转录因子。第三个目标侧重于共生细菌在宿主生殖过程中不可或缺的作用。它将表征共生体为促进宿主繁殖力提供的因素，分析共生体治愈的不育果蝇中宿主繁殖相关基因的表达模式。总的来说，拟议的研究将确定采采蝇繁殖过程所需的关键要素。这些要素将为用于预防锥虫病的采采蝇特定病媒控制策略提供目标。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Rapid autophagic regression of the milk gland during involution is critical for maximizing tsetse viviparous reproductive output.

复旧过程中乳腺的快速自噬消退对于最大化采采蝇胎生繁殖输出至关重要。

DOI：
发表时间：
2018
期刊：
影响因子：
3.8
作者：
Benoit, Joshua B;Michalkova, Veronika;Didion, Elise M;Xiao, Yanyu;Baumann, Aaron A;Attardo, Geoffrey M;Aksoy, Serap
通讯作者：
Aksoy, Serap

A comparative analysis of reproductive biology of insect vectors of human disease.

人类疾病昆虫媒介生殖生物学的比较分析。

DOI：
发表时间：
2015-08-01
期刊：
影响因子：
0
作者：
Shaw, W Robert;Attardo, Geoffrey M;Aksoy, Serap;Catteruccia, Flaminia
通讯作者：
Catteruccia, Flaminia

Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia: transcriptomic and metabolomic landscapes reveal highly integrated physiological networks.

揭示采采蝇与其专性共生体 Wigglesworthia 之间的关系：转录组学和代谢组学景观揭示了高度整合的生理网络。

DOI：
发表时间：
2017-06-28
期刊：
影响因子：
0
作者：
Bing, XiaoLi;Attardo, Geoffrey M;Vigneron, Aurelien;Aksoy, Emre;Scolari, Francesca;Malacrida, Anna;Weiss, Brian L;Aksoy, Serap
通讯作者：
Aksoy, Serap

Lipophorin acts as a shuttle of lipids to the milk gland during tsetse fly pregnancy.