A Xenopus Laevis Research Resource for Immunobiology

爪蟾免疫生物学研究资源

基本信息

批准号：
8739925
负责人：
JACQUES Robert
金额：
$ 29.69万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-06-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8739925
关键词：
Address Adult Advertisements Amphibia Animal Model Animals Antigens Antiviral Agents Autoimmunity Awareness Biological Metamorphosis Biology Biomedical Research Breeding CD8B1 gene Cell Line Cells Communicable Diseases Communities Databases Development Developmental Biology Embryo Evolution Expressed Sequence Tags Fibroblasts Funding Gene Expression Gene Transfer Techniques Generations Genes Genetic Genome Goals Grant Growth Health Histocompatibility Antigens Class I Human Immune Immune response Immune system Immunity Immunobiology Immunological Models Immunology Inbred Strain Inbreeding Inflammation Laboratories Larva Lead Maintenance Malignant Neoplasms Mammals Mediating Medical Methodology Modeling Monoclonal Antibodies Organ Outcomes Research Performance Phylogeny Physiological Physiology Productivity Proteins RNA RNA Interference Rana Ranavirus Reagent Recombinants Reporter Reporter Genes Research Resources Scientist Self Tolerance Services Site Staging Students Study models System T-Lymphocyte Tadpoles Techniques Technology Time Tissues Training Transgenic Animals Transgenic Organisms Tumor Cell Line United States National Institutes of Health Update Viral Wood material Xenopus Xenopus laevis base comparative endodeoxyribonuclease SceI genetic resource immune function improved innovation insight interest intravital microscopy investigator training loss of function lymphoid neoplasm macrophage new technology novel strategies promoter public health relevance research and development research facility small hairpin RNA therapy development tool tumor web site

项目摘要

DESCRIPTION (provided by applicant): The goal of this renewal application is to continue maintaining and developing the world's most comprehensive research resource specializing in the use of the amphibian Xenopus laevis for biomedical and immunological research. Interests and relevance of X. laevis for human health are due to the remarkable similarity of its immune system with that of human, the accessibility to experimentation at all developmental stages, as well as the availability of large genetic resources, invaluable MHC-defined inbred strains and clones of frogs and tools such as fibroblast and lymphoid tumor cell lines, monoclonal antibodies, MHC tetramers and batteries of validated PCR primers for immune-relevant genes. These animals and reagents need to be preserved, enriched, and made available to the scientific community. The broad objective of this renewal application is to continue managing, safeguarding, promoting and further developing X. laevis as a unique multi-faceted experimental platform for research in fundamental and medical immunology and for the benefit of the whole scientific community. As in previous proposals, two major aims are proposed: (1) Maintenance, improvement and advertisement of our X. laevis facility by continuing to maintain and improve the performance and quality of our resource; by providing animals and reagents, not commercially available, upon request; by assisting, training, and informing scientists and students about X. laevis; and by promoting information, accessibility and public awareness of the resource through a frequently updated website connected with other important Xenopus resources in US and in the world as well as with sites involved with immunology, amphibian and conservation biology. (2) Development of new experimental animals, methodologies, and reagents by generating transgenic (Tg) X. laevis inbred lines and clones with RNA-mediated loss-of-function, new fluorescent Tg reporter inbred MHC-defined lines and clones, new reagents (monoclonal antibodies, MHC tetramers), and by developing X. laevis tadpoles for real time intravital microscopy using fluorescent ranavirus recombinants, tumor transfectants and X. laevis transgenic reporter lines to visualize antiviral and antitumor immune responses. In addition to maintaining a research platform that is crucial for the Xenopus scientific community, this project promotes the development of new approaches and technologies that can be rapidly and broadly applied for innovative insight into tissue and organ physiology, immunology and developmental biology. The development and application of these tools and technologies will contribute to the efforts of the Xenopus community assisted by the NIH to establish Xenopus as a relevant model for biomedical research. Given the high degree of genetic and functional similarities between Xenopus and mammalian immune and other physiological systems, it is anticipated that outcomes from research promoted by the X. laevis Research Resource will lead to applications relevant to human related research and therapy development.

描述（由申请人提供）：本次更新申请的目标是继续维护和开发世界上最全面的研究资源，专门利用两栖动物非洲爪蟾进行生物医学和免疫学研究。 X. laevis 对人类健康的兴趣和相关性是由于其免疫系统与人类的免疫系统显着相似，在所有发育阶段均可进行实验，以及大量遗传资源的可用性，MHC 定义的无价近交菌株青蛙克隆和成纤维细胞和淋巴肿瘤细胞系、单克隆抗体、MHC 四聚体以及经过验证的免疫相关基因 PCR 引物组等工具。这些动物和试剂需要保存、丰富并提供给科学界。这一更新应用程序的总体目标是继续管理、保护、促进和进一步开发 X. laevis，将其作为基础和医学免疫学研究的独特多方面实验平台，并造福整个科学界。与之前的提案一样，提出了两个主要目标：（1）通过继续维持和提高我们资源的性能和质量，对我们的 X. laevis 设施进行维护、改进和广告；根据要求提供非市售的动物和试剂；通过协助、培训科学家和学生并向他们介绍 X. laevis；通过与美国和世界其他重要非洲爪蟾资源以及涉及免疫学、两栖动物和保护生物学的网站相连的经常更新的网站，促进资源的信息、可访问性和公众意识。 (2) 通过产生具有RNA介导的功能丧失的转基因(Tg) X. laevis自交系和克隆、新的荧光Tg报告基因近交系和MHC定义的克隆、新试剂来开发新的实验动物、方法和试剂（单克隆抗体、MHC 四聚体），并使用荧光蛙病毒重组体、肿瘤转染子和非洲爪蟾开发用于实时活体显微镜检查的非洲爪蟾蝌蚪转基因报告系可视化抗病毒和抗肿瘤免疫反应。除了维护对非洲爪蟾科学界至关重要的研究平台外，该项目还促进新方法和技术的开发，这些方法和技术可以快速广泛地应用于组织和器官生理学、免疫学和发育生物学的创新见解。这些工具和技术的开发和应用将有助于在 NIH 的协助下，非洲爪蟾社区将非洲爪蟾建立为生物医学研究的相关模型。鉴于非洲爪蟾与哺乳动物免疫和其他生理系统之间的遗传和功能高度相似，预计非洲爪蟾研究资源推动的研究成果将导致与人类相关研究和治疗开发相关的应用。