Bioanalytical Core

生物分析核心

• 批准号: 8882092
• 负责人: ARIEL Y DEUTCH
• 金额: $ 18.01万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8882092
• 关键词: Area; Autoradiography; Behavior; Binding; Biochemical; Biological Assay; Brain; Communities; Development; Environment; Genotype; Goals; In Vitro; Investigation; Laboratories; Life; Measurement; Measures; Membrane; Methodology; Methods; Microdialysis; Molecular; Molecular Genetics; Mus; Neurons; Neurosciences Research; Neurotransmitters; Pharmacology; Physiological; Physiology; Proteins; Psychiatry; Research; Research Personnel; Research Support; Resources; Scientist; Serotonin; Signal Transduction; Slice; Training; awake; experience; extracellular; in vivo; instrumentation; neurochemistry; neurotransmitter release; professor; radioligand; receptor; skills; web site

The Vanderbilt Conte Center for Neuroscience Research Enduring Impact of Early-Life Serotonin Signaling involves a set of highly interdependent projects targeting genetic, molecular, biochemical and cellular facets of serotonin (5-HT) neuronal identity, development, signaling, physiology and behavior. The Bioanalytical Core provides a set of physical resources in dedicated space accompanied by dedicated technical staff to support the efforts of Conte Center projects. The Bioanalytical Core, under the directorship of Dr. Ariel Deutch, Professor of Psychiatry and Pharmacology at Vanderbilt, organizes research support on behalf of Conte Investigators in the areas of neurochemical, receptor, and transport assays, protein analyses, and mouse genotyping. Neurochemical analyses include RIA assays to measure signal transduction molecules and neurotransmitter release assays using in vivo microdialysis or in vitro slice suprafusion approaches. Receptor assays include radioligand binding to isolated brain membranes or autoradiography analysis of mouse brain sections. The Core also provides support for the genotyping of mouse lines used in each of the projects. Research space dedicated to core activities in the MRBIII building contains instrumentation and bench space for daily activities related to support activities and includes a microdialysis laboratory room to provide a quiet environment for the measurement of extracellular neurotransmitters in awake, freely moving mice. As with our prior application, Drs. Deutch and Gresch coordinate research support activities with the ongoing pace and priorities of Conte projects and provide training of junior scientists to gain experience in laboratory methodologies that can facilitate their professional development. The detailed methods and resources offered by the Core are shared with Conte investigators and the scientific community through the Conte website and demonstrated through one-on-one interactions with project staff. Finally, the Core provides support for Conte pilot grantees to insure the optimum use of pilot resources and the progression of projects toward more sustained research support.

范德比尔特康特神经科学研究中心的早期血清素信号传导的持久影响涉及一系列高度相互依赖的项目，针对血清素 (5-HT) 神经元身份、发育、信号传导、生理学和行为的遗传、分子、生化和细胞方面。生物分析核心在专用空间中提供了一组物理资源，并配有专门的技术人员，以支持 Conte 中心项目的工作。生物分析核心在范德比尔特大学精神病学和药理学教授 Ariel Deutch 博士的领导下，代表 Conte 研究者在神经化学、受体和转运测定、蛋白质分析和小鼠基因分型领域组织研究支持。神经化学分析包括测量信号转导分子的 RIA 测定和使用体内微透析或体外切片超融合方法的神经递质释放测定。受体测定包括放射性配体与分离的脑膜的结合或小鼠脑切片的放射自显影分析。该核心还为每个项目中使用的小鼠品系的基因分型提供支持。 MRBIII 大楼内专门用于核心活动的研究空间包含用于与支持活动相关的日常活动的仪器和工作台空间，并包括一个微透析实验室房间，为测量清醒、自由活动的小鼠的细胞外神经递质提供安静的环境。与我们之前的申请一样，博士。多伊奇和格雷施根据 Conte 项目的持续节奏和优先事项协调研究支持活动，并为初级科学家提供培训，以获得实验室方法方面的经验，从而促进他们的专业发展。核心提供的详细方法和资源通过 Conte 网站与 Conte 研究人员和科学界共享，并通过与项目工作人员的一对一互动进行演示。最后，核心为 Conte 试点受资助者提供支持，以确保试点资源的最佳利用以及项目向更持续的研究支持的进展。