Flow Cytometry

流式细胞仪

• 批准号: 9149774
• 负责人: Jianzhu Chen
• 金额: $ 25.02万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-09 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9149774
• 关键词: Apoptosis; Budgets; Cancer Center; Cancer Center Support Grant; Cell Cycle Progression; Cell Separation; Cells; Color; Complex; Containment; Core Grant; Custom; Data Analyses; Development; Engineering; Flow Cytometry; Funding; Human; Imaging technology; Institutes; Label; Lasers; Love; Mission; Population; Proteins; Research Personnel; Research Support; Resource Sharing; Running; Sampling; Services; Sorting - Cell Movement; Speed; Systems Development; Technical Expertise; Technology; Training; anticancer research; color detection; cost; design; instrument; instrumentation; investigator training; magnetic beads; member; new technology; novel; particle; programs; response; success; tool; trend; tumor

Flow cytometry is a critical tool for cancer research. It is used to identify, track and purify specific cell populations, assess cell cycle progression and apoptosis responses, and to support a broad range of engineering approaches such as the quantification of delivery systems and the development of novel protein labeling and imaging technologies. The Koch Institute Flow Cytometry Core is a Shared Resource that provides Center Members training in, and access to, state-of-the-art instrumentation, data analysis and technical expertise in cell sorting and analyses. In the current period, the capabilities of this Core were further expanded and enhanced. This includes moving into a larger, custom-designed space in the new Koch Institute building, an increase in the Core staff and the acquisition of new instrumentation. Notably, in the same period, there has been a substantial increase in the number of Center Members who use the Flow Cytomtery Core from 78% to 94%, including investigators from all four Programs. Thus, this Shared Resource is essential to the success of the Koch Institute mission. In the upcoming period, the Flow Cytometry Core will continue to offer a wide range of state-of-the-art services to support the research programs of Center Members. Additionally, the Core plans to make new instrument purchases, with the support of MIT funds, and establish cutting edge single cell technologies, with the support of requested developmental funds. In spite of the major expansion in services and the resulting increased costs of running this Core, the requested CCSG budget for Year 44 is slightly reduced from the requested and recommended budget in Year 39.

流式细胞仪是癌症研究的关键工具。它用于识别，跟踪和净化特定的单元 种群，评估细胞周期进展和凋亡反应，并支持广泛的 工程方法，例如量化输送系统和新蛋白质的发展 标签和成像技术。 Koch Institute流式细胞仪核心是共享资源 提供中心成员在最先进的仪器，数据分析和访问中的培训和访问 细胞分类和分析方面的技术专长。在当前时期，该核心的功能进一步 扩展和增强。这包括进入新科赫研究所的更大，定制设计的空间 建造，核心人员的增加以及新仪器的收购。值得注意的是，在同一时期 使用流式细胞瘤核心的中心成员数量已大大增加 从78％到94％，包括来自所有四个计划的调查人员。因此，此共享资源对于 科赫研究所任务的成功。在接下来的时期，流式细胞仪核心将继续 提供各种最先进的服务，以支持中心成员的研究计划。 此外，在麻省理工学院基金的支持下进行新工具的核心计划，并建立 尖端的单细胞技术，并在要求的开发基金的支持下。尽管有专业 服务的扩展以及运行此核心的成本增加，要求的CCSG预算 第44年从39年的要求和建议预算略有减少。