CELL IMAGING AND HISTOPATHOLOGY

细胞成像和组织病理学

基本信息

批准号：
8378391
负责人：
BARBARA RANSCHT
金额：
$ 26.86万
依托单位：
SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-21 至 2015-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8378391
关键词：
Address Algorithms Apoptosis Budgets Cancer Center Cancer Center Support Grant Cell Communication Cells Cellular Structures Communication Computer software Computers and Advanced Instrumentation Consult Consultations Data Data Analyses Dependence Detection Development Disease Dissection Documentation Electron Microscope Electron Microscopy Electrons Endocytosis Environment Equipment Event Exocytosis Experimental Genetics Experimental Models Fluorescence Resonance Energy Transfer Generations Genes Genetic Genetic Models Histology Histopathology Histopathology Facility Housing Human Image Image Analysis Imaging Device Imaging Techniques Immunohistochemistry Institutes Internet Investigation Label Lasers Life Location Malignant Neoplasms Microscope Microscopic Mission Molecular Molecular Probes Monitor Movement Mus Names Normal Cell Organelles Pathologist Patients Persons Population Preparation Process Proteins Protocols documentation Qualifying Research Research Personnel Research Support Resolution Resource Sharing Resources Scanning Science Services Signal Transduction Slide Specimen Staining method Stains System Techniques Technology Time Tissue Microarray Tissue Sample Tissues Training Training Support Work cancer cell carcinogenesis cell motility cellular imaging cost effective data acquisition data sharing design digital drug modification experience fluorescence microscope image archival system improved instrument instrumentation investigator training laser capture microdissection member molecular imaging molecular pathology molecular/cellular imaging mouse model multi-photon receptor research study response tissue culture tissue processing tool transmission process

项目摘要

Observations of molecular changes in patient tissues or caused by genetic or experimental manipulations in mice and cancer cells in culture critically depend on histological and immunohistological analyses in cells and tissues through advanced imaging techniques. The mission of the Cell Imaging and Histopathology Shared Resource is to provide state-of-the-art imaging and histopathology tools, training, and services to Cancer Center researchers. The Shared Resource is divided into two adjacent Facilities: 1) The Cell /mag/ng facility houses four confocal microscopes and five additional fluorescence microscopes. All the microscopes are equipped with advanced digital cameras and image analysis software. Available systems include laser scanning confocal microscopes with single and multi-photon lasers, as well as a spinning disk confocal microscope for extended live cell imaging. Time-lapse imaging systems are available to study cell motility, proliferation, and differentiation, with FRET and TIRF systems to image intracellular dynamic interactions. A full range of wide-field imaging approaches are also supported. The facility transmission electron microscope service provides analysis of cell and tissue ultrastructure. Facility staff maintains the imaging equipment and provides investigator training and support for imaging and image analysis, as well as imaging service, and active development of new imaging tools. 2) The Histopathology facility offers the preparation, sectioning, staining and analysis of tissue samples for histological and histopathological analyses and probe-specific detection of cellular and molecular changes in tissue sections. For analysis of identified cells or cell groups within tissue sections, the facility offers Laser Capture Microdissection services. An automated slide scanning system affords efficient histological data acquisition, image archiving, analysis and quantitation. A staff pathologist with expertise in both human and mouse specimens provides consultation, as well as assistance with tissue microarrays. The Facility staff provides consultation, user training, development and dissemination of imaging-, histology- and immunohistochemical protocols, and assistance with instrumentation for efficient histology data documentation, analysis and storage. The Shared Resource supported the studies of 35 Cancer Center Members in the past year, and is essential to advance the understanding of molecular and cellular events leading to cancer development and spreading. Overall, $185,234 in CCSG support is requested in the first year, representing 18.4% of the total projected annual operating budget.

观察患者组织分子变化或由遗传或实验操作引起的观察培养物中的小鼠和癌细胞严重取决于细胞中的组织学和免疫组织学分析和组织通过高级成像技术。细胞成像和组织病理学的任务共享资源是提供最先进的成像和组织病理学工具，培训和服务癌症中心的研究人员。共享资源分为两个相邻的设施： 1）细胞 /mag /ng设施包含四个共聚焦显微镜和五个额外的荧光显微镜。所有显微镜都配备了高级数码相机和图像分析软件。可用的系统包括带有单个和多光子激光器的激光扫描共聚焦显微镜以及用于扩展活细胞成像的旋转磁盘共聚焦显微镜。有延时成像系统可用研究细胞运动，增殖和分化，并使用FRET和TIRF系统来对细胞内进行图像动态相互作用。还支持各种宽场成像方法。设施透射电子显微镜服务提供了细胞和组织超微结构的分析。设施人员维护成像设备，并为成像和图像提供研究者的培训和支持分析以及成像服务以及新成像工具的积极开发。 2）组织病理学设施提供了组织样品的准备，切片，染色和分析组织学和组织病理学分析以及探针特异性检测细胞和分子变化组织切片。为了分析组织切片内已识别的细胞或细胞组，该设施提供激光捕获微分解服务。自动幻灯片扫描系统提供有效的组织学数据获取，图像归档，分析和定量。具有人类专业知识的人员病理学家小鼠标本提供咨询，并为组织微阵列提供帮助。设施人员提供咨询，用户培训，开发和传播成像，组织学和免疫组织化学方案和有效组织学仪器的帮助文档，分析和存储。共享资源支持35个癌症中心的研究在过去的一年中，成员对于促进分子和细胞事件的理解至关重要导致癌症发展和扩散。总体而言，第一次要求$ 185,234的CCSG支持年，占预计年度运营预算总计的18.4％。