Core--Tissue culture

核心--组织培养

• 批准号: 6583740
• 负责人: BARBARA RANSCHT
• 金额: $ 20.05万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6583740
• 关键词: biomedical facility; hippocampus; histology; tissue /cell culture; transfection

Description: This core consists of a facility that assists all participating laboratories with tissue culture experiments, and provides assistance with histological experiments. Tissue culture will be used in all four components of this program to analyze the role and mechanism of cell adhesion molecules (Ranscht), receptor protein tyrosine kinases (Pasquale) and cell surface proteoglycans (Stallcup and Yamaguchi) by cell transfection assays and/or assays for neurite growth, synaptogenesis, and spine formation. The Tissue Culture will assist investigations of this Program Project with the transfection of cDNA constructs in cell lines and primary neural cultures, and setup primary cultures from the hippocampus to assay neuronal differentiation and synaptogenesis. One major advantage of this core is that the hippocampal culture system is standardized, so that results obtained for one of the studied molecules can be related to those for others. This will broaden the understanding of the molecular nature of neuronal differentiation and synaptogenesis, and foster additional interactions and joint experiments between the program project laboratories. The Core will also provide assistance with histological experiments by cutting frozen and paraffin sections, and perform immunostaining with various antibodies.

描述：该核心由一个设施组成，该设施可以帮助所有参与的实验室进行组织培养实验，并为组织学实验提供帮助。组织培养将用于该程序的所有四个组成部分，以分析细胞粘附分子（Ranscht），受体蛋白酪氨酸激酶（Pasquale）（Pasquale）和细胞表面蛋白聚糖（Stallcup和Yamaguchi）的作用和机制，并通过细胞转染调查和/或分析通过神经元素生长，并构成，并构成了细胞转移分析和/或分析。组织培养将通过在细胞系和原发性神经培养物中转染cDNA构建体的转染，并从海马培养物中进行cDNA构建体的转染，从海马到测定神经元分化和突触发生。该核心的主要优点是标准化海马培养系统，因此，研究分子之一获得的结果可能与其他分子有关。这将扩大对神经元分化和突触发生的分子性质的理解，并促进程序项目实验室之间的其他相互作用和联合实验。核心还将通过切割冷冻和石蜡切片并用各种抗体进行免疫染色来为组织学实验提供帮助。