Phase variable control of Acinetobacter baumannii virulence

鲍曼不动杆菌毒力的相变量控制

• 批准号: 8809155
• 负责人: Philip N. Rather
• 金额: $ 19.12万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8809155
• 关键词: Acinetobacter baumannii; Address; Antibiotics; Binding; Cell Shape; Cells; Cellular Morphology; Clinical; Defect; Development; Event; Exhibits; Feedback; Frequencies; Gene Expression; Genes; Genetic; Goals; Gram-Negative Bacteria; Human; In Vitro; Infection; Mediating; Molecular; Molecular Profiling; Morphology; Mutation; Nosocomial Infections; Phase; Phenotype; Production; RNA; Regulation; Research Personnel; Signal Transduction; Surface; Testing; Variant; Virulence; Work; base; cell motility; combat; genome sequencing; in vivo; novel; public health relevance; quorum sensing; resistant strain; response

 DESCRIPTION (provided by applicant): The Gram-negative bacterium Acinetobacter baumannii is a leading cause of nosocomial infections in humans. The emergence of A. baumannii strains resistant to most, and in some cases, all available antibiotics has made the treatment of these infections exceedingly difficult. This underlies the importance of finding new ways to combat these infections. This proposal addresses a phase variable mechanism in A. baumannii that controls multiple phenotypes, including colony opacity, quorum sensing and virulence. Using a combination of allelic replacement and whole genome sequencing, the mechanism responsible for this phase variation will be determined. The impact of phase variation on global gene expression will also be determined using RNA- Seq. Ultimately, an understanding of the molecular mechanisms that control this phase variation and the overall phenotypes impacted by this variation will provide important information on the regulation of virulence in A. baumannii.

 描述（由申请人提供）：革兰氏阴性细菌鲍曼不动杆菌是人类医院感染的主要原因，鲍曼不动杆菌菌株的出现对大多数抗生素、在某些情况下对所有可用抗生素都具有耐药性，这使得这些感染的治疗成为可能。这表明寻找新方法来对抗这些感染的重要性。该提案解决了鲍曼不动杆菌中控制多种表型（包括菌落）的相变机制。结合等位基因替换和全基因组测序，将确定相位变异对整体基因表达的影响。了解控制这种相变的分子机制以及受这种变化影响的总体表型将为鲍曼不动杆菌毒力的调节提供重要信息。