Functions of conditional Cx36-dependent electrical synapses in the neocortex

新皮质中条件性 Cx36 依赖性电突触的功能

• 批准号: 8525570
• 负责人: Arthur U. Sugden
• 金额: $ 4.22万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8525570
• 关键词: Affect; Agonist; Antimalarials; Attention; Behavior; Brain; Cells; Cerebral cortex; Charge; Chronic; Connexins; Coupled; Coupling; D Cells; Disease; Electrical Synapse; Electrodes; Electroencephalogram; Electroencephalography; Epilepsy; Frequencies; Gap Junctions; Goals; Hallucinations; Hippocampus (Brain); In Vitro; Interneurons; Juvenile Myoclonic Epilepsy; Knock-out; Link; Measures; Mediating; Mefloquine; Mental disorders; Mus; Mutation; Neocortex; Neurons; Optics; Parvalbumins; Pharmaceutical Preparations; Phase; Play; Prosencephalon; Pyramidal Cells; Ramp; Role; Schizophrenia; Sensory; Series; Signal Transduction; Slice; Somatosensory Cortex; Somatostatin; Tamoxifen; Testing; Thalamic structure; Theta Rhythm; Time; Visual Cortex; Visual attention; Whole-Cell Recordings; arm; base; cell type; connexin 36; excitatory neuron; expectation; gap junction channel; genome wide association study; in vivo; neocortical; nervous system disorder; optogenetics; promoter; public health relevance; recombinase; response; tool

DESCRIPTION: Cortical rhythms have been implicated in attention, exploratory and navigatory behavior, and in disease states such as schizophrenia, but their mechanisms remain vague. One clue to their origin is that neuronal gap junction expression is required for full power in the gamma band in the hippocampus. Gap junctions synchronize neighboring inhibitory interneurons of the same subtype but their role in network activity remains poorly understood. I propose to investigate the interactions between two gap-junction connected networks of interneurons, somatostatin-expressing interneurons and parvalbumin-expressing interneurons, and two types of cortical oscillations, theta (4-12 Hz) and gamma (30-80 Hz). I will investigate this link by selectively eliminating connexin36-based gap junctions in the two interneuronal networks and measuring the consequences on gamma and theta rhythms generated in vitro with whole-cell recordings and in vivo via electroencephalography and laminar electrode arrays.

描述：皮质节律与注意力、探索和导航行为以及精神分裂症等疾病状态有关，但其机制仍然模糊。它们起源的一条线索是，神经元间隙连接表达是神经元发挥全部功能所必需的。 海马体中的伽玛带。间隙连接使相同亚型的相邻抑制性中间神经元同步，但它们在网络活动中的作用仍然知之甚少。我建议研究两个间隙连接连接的中间神经元网络、表达生长抑素的中间神经元和表达小白蛋白的中间神经元，以及两种类型的皮质振荡，theta (4-12 Hz) 和 gamma (30-80 Hz) 之间的相互作用。我将通过选择性消除两个神经元网络中基于 connexin36 的间隙连接来研究这种联系，并通过全细胞记录测量体外产生的 γ 和 θ 节律的影响，以及通过脑电图和层状电极阵列测量体内产生的 γ 和 θ 节律的影响。