Agilent 6490 Triple Quadrupole Mass Spectrometer

Agilent 6490 三重四极杆质谱仪

• 批准号: 8640509
• 负责人: JULIE D SABA
• 金额: $ 52.34万
• 依托单位: CHILDREN'S HOSPITAL & RES CTR AT OAKLAND
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640509
• 关键词: Amino Acids; Binding Proteins; Biological Markers; Cardiovascular Diseases; Cells; Clinical; Complex; Core Facility; Coupled; Detection; Diabetes Mellitus; Disease; Drug Delivery Systems; Equipment; Funding; Genetic; Health; High Pressure Liquid Chromatography; Immunology; Inflammation; Institutes; Lipid Binding; Lipids; Lipoproteins; Liquid Chromatography; Longevity; Metabolism; Methods; Nanotechnology; Nutrient; Pediatric Hospitals; Pediatric Research; Performance; Pharmaceutical Preparations; Pharmacologic Substance; Phospholipids; Proteins; Recruitment Activity; Reproducibility; Request for Proposals; Research; Research Institute; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Sickle Cell Anemia; Sphingolipids; System; Technology; Thalassemia; Time; United States National Institutes of Health; Vitamin D; anticancer research; arm; experience; innovation; instrument; instrumentation; mass spectrometer; metabolomics; millisecond; multiple reaction monitoring; nutrition; obesity prevention; programs; ultra high pressure; vaccine development

DESCRIPTION (provided by applicant): Children's Hospital Oakland Research Institute (CHORI) is a free-standing institute that serves as the research arm of the Children's Hospital & Research Center Oakland. Our research program is among the top pediatric research departments in the nation with regard to funding from the National Institutes of Health (NIH). Research is heavily focused on lipids and lipoproteins in health and disease. This proposal requests funds to purchase an Agilent 6490 Triple Quadrupole mass spectrometer (MS) equipped with 1290 Infinity Quaternary liquid chromatography (LC) system. This LC/triple quadrupole MS combined system would replace an out-of- date LC/MS system that is rapidly approaching the end of its lifespan. LC/MS instrumentation is a critical resource for a large and diverse array of research projects involving metabolomics, analysis of biomarkers of disease and inflammation, drug delivery using nanotechnology, lipid quantitation, and analysis of lipid-binding proteins. The instrument would be used to quantify target molecules including sphingolipids, vitamin D, amino acid metabolites, thiolated and oxidized proteins, phospholipids, oxysterols and metabolites of statin drugs. Twelve major users representing a cross-section of CHORI's major scientific centers, including Cancer Research; Sickle Cell Disease & Thalassemia; Nutrition & Metabolism; Immunology & Vaccine Development; Prevention of Obesity, Cardiovascular Disease & Diabetes; and Genetics will be supported by the instrument. The 6490, with iFunnel Technology, enables zeptomole detection limits at conventional HPLC flow rates. The 6490 instrument provides unsurpassed sensitivity for the analysis of pharmaceutical compounds, trace-level nutrients, clinical samples, drugs, metabolites, and biomarkers. With 1 millisecond dwell times and no collision cell cross talk, the 6490 QqQ is ideally coupled with the 1290 ultra high-pressure/performance LC (UHPLC) system for fast sample analyses, allowing reliable peak integration and quantitation across even 1 second-wide UPHLC peaks. For very complex sample mixtures, the 6490 QqQ allows dynamic multiple reaction monitoring (MRM) methods with up to 4,000 transitions per method and polarity switching in as little as 30 milliseconds. The Agilent 6490 QqQ is a versatile, state-of-the-art instrument with high sensitivity, linearity, dynamic range, reproducibility and robustness and which represents a technology with which our user group is highly familiar. Thus, we have chosen this as the optimal system from a number of competing MS systems. The new LC/MS system will significantly advance the current projects and add to the pool of shared equipment at CHORI. CHORI's scientists are adept at LC/MS; we have long-standing experience in pioneering MS technology and maintaining an active MS Core Facility. We have also recruited external scientific advisors to further insure the instrument is utilized properly and to the highet capacity possible for innovative research by the largest number of CHORI investigators and potentially by external investigators through collaborative research endeavors.

描述（由申请人提供）：奥克兰儿童医院研究所（Chori）是一家独立的研究所，是奥克兰儿童医院与研究中心的研究部门。关于美国国立卫生研究院（NIH）的资助，我们的研究计划是美国顶级的儿科研究部门之一。研究重点是健康和疾病中的脂质和脂蛋白。该提案要求资金购买配备1290个Infinity Quaternary液体色谱（LC）系统的Agilent 6490三倍四极杆质谱仪（MS）。这个LC/三倍四极杆MS组合系统将取代已迅速接近其寿命结束的旧约LC/MS系统。 LC/MS仪器是涉及代谢组学，疾病和炎症生物标志物的大量研究项目的关键资源，使用纳米技术，脂质定量和脂质结合蛋白的分析。该仪器将用于量化靶标分子，包括鞘脂，维生素D，氨基酸代谢产物，硫醇和氧化蛋白，磷脂，氧蛋白酶，氧甲醇和他汀类药物的代谢产物。十二位主要用户，代表了合唱的主要科学中心的横截面，包括癌症研究；镰状细胞病和丘脑贫血；营养与新陈代谢；免疫学和疫苗开发；预防肥胖，心血管疾病和糖尿病；遗传学将得到该工具的支持。具有ifunnel技术的6490可以在常规的HPLC流速下实现Zeptomole检测极限。 6490仪器为分析药物化合物，痕量水平营养素，临床样品，药物，代谢物和生物标志物提供了无与伦比的灵敏度。在1毫秒的停留时间和无碰撞细胞串讨论的情况下，6490 QQQ与1290 Ultra高压/性能LC（UHPLC）系统相结合，进行快速样品分析，从而可以在1秒宽的UPHLC上进行可靠的峰值整合和定量峰。对于非常复杂的样品混合物，6490 QQQ允许动态多重反应监测（MRM）方法，每种方法最多4,000个过渡，并且极性切换仅为30毫秒。 Agilent 6490 QQQ是一种多功能，最先进的仪器，具有高灵敏度，线性，动态范围，可重复性和鲁棒性，它代表了我们用户组非常熟悉的技术。因此，我们从许多竞争的MS系统中选择了它作为最佳系统。新的LC/MS系统将大大推动当前项目，并增加Chori共享设备的池。 Chori的科学家擅长LC/MS；我们在开创MS技术并维护主动MS核心设施方面拥有长期的经验。我们还招募了外部科学顾问，以进一步确保正确利用该工具，并通过最多的Chori研究人员以及外部研究人员通过协作研究的努力来实现创新研究的能力。

项目成果

Efficacy of AAV9-mediated SGPL1 gene transfer in a mouse model of S1P lyase insufficiency syndrome.

AAV9 介导的 SGPL1 基因转移在 S1P 裂解酶不足综合征小鼠模型中的功效。

• DOI: 10.1172/jci.insight.145936
• 发表时间: 2021
• 期刊: JCI insight
• 影响因子: 8
• 作者: Zhao,Piming;Tassew,GizachewB;Lee,JoannaY;Oskouian,Babak;Muñoz,DeniseP;Hodgin,JeffreyB;Watson,GordonL;Tang,Felicia;Wang,Jen-Yeu;Luo,Jinghui;Yang,Yingbao;King,Sarah;Krauss,RonaldM;Keller,Nancy;Saba,JulieD
• 通讯作者: Saba,JulieD

Randomized nutrient bar supplementation improves exercise-associated changes in plasma metabolome in adolescents and adult family members at cardiometabolic risk.

• DOI: 10.1371/journal.pone.0240437
• 发表时间: 2020
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Mietus-Snyder M;Narayanan N;Krauss RM;Laine-Graves K;McCann JC;Shigenaga MK;McHugh TH;Ames BN;Suh JH
• 通讯作者: Suh JH