Nitrogen Metabolism in Aedes Aegypti Mosquitoes

埃及伊蚊的氮代谢

基本信息

批准号：
8676639
负责人：
Patricia Yolanda Scaraffia
金额：
$ 37.64万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-06 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8676639
关键词：
Address Adult Aedes Affect Amides Amino Acids Ammonia Amphibia Applications Grants Arginine Assimilations Bacteria Biochemical Biochemical Process Biology Blood Carbon Carbon Dioxide Cessation of life Citric Acid Cycle Culicidae Data Deamination Dengue Detoxification Process Digestion Disease Double-Stranded RNA Drug Metabolic Detoxication Drug resistance Economics Essential Amino Acids Excretory function Female Fishes Funding Genes Glucose Glutamine Insecta Insecticide Resistance Invertebrates Isotope Labeling Keto Acids Knowledge Label Lead Link Malaria Mass Spectrum Analysis Metabolic Metabolic Pathway Metabolism Methodology Methods Molecular Biology Monitor Mosquito Control Nitrogen Nutrient Organism Outcome Parasites Pathway interactions Physiological Physiological Processes Plants Population Process Proteins RNA Interference Regulation Reporting Risk Route Signal Transduction Skeleton Techniques Time Tissues Urate Oxidase Urea Uric Acid Vertebrates Weight West Nile virus Yellow fever virus arginase base biological systems chikungunya coping design feeding glucose metabolism knock-down mass spectrometer metabolomics nitrogen metabolism operation oxidation prevent research study sample fixation small molecule tool vector mosquito wasting

项目摘要

DESCRIPTION (provided by applicant): We have reported that Aedes aegypti female mosquitoes possess a remarkable biological system to survive toxic ammonia concentrations released by blood meal digestion using previously unknown metabolic pathways present in this organism. Specifically, we have found that Ae. aegypti can detoxify ammonia by the same metabolic pathways that nitrogen fixing plants and bacteria use, unlike all other insects that have been studied. Mosquitoes can also use the nitrogen from the amide group of glutamine to produce uric acid as vertebrates do, and more surprisingly, mosquitoes hydrolyze uric acid into urea through the same metabolic pathway that some fish and amphibians use. Since the coordinated operation of these multiple metabolic pathways prevents a lethal ammonia concentration in the mosquito tissues after a blood meal, it impels us to know how these metabolic pathways are interconnected and regulated. Therefore, the main purpose of the grant application is to elucidate the metabolic regulation of nitrogen waste, as well as to analyze the flux of C and N molecules involved in ammonia metabolism in mosquitoes. These studies will be performed using a combination of RNA interference and mass spectrometry techniques. Attempts to control mosquito populations using biorational approaches depend on a thorough understanding of mosquito biology and metabolism. So, if the experiments that we propose are conducted successfully, they will open up new and exciting discoveries that could be applied for the design and implementation of better strategies for mosquito control. The following specific aims will be pursued: 1- Identify the mechanisms involved in the regulation of nitrogen waste in mosquitoes. 2- Investigate the flux of C and N molecules involved in ammonia metabolism in mosquitoes. According to our preliminary data, the metabolic regulation of nitrogen waste appears to be tightly- regulated in Ae. aegypti mosquitoes. Therefore, our objective is to understand how this regulatory mechanism controls the ammonia metabolism in adult female mosquitoes.

描述（由申请人提供）：我们报道说，埃及埃及女性蚊子具有出色的生物系统，可以使用该生物体中存在的先前未知的代谢途径来生存由血液粉消化释放的有毒氨浓度。具体来说，我们发现了AE。与所有已研究的其他昆虫不同，埃及可以通过固定植物和细菌的氮固定植物和细菌的代谢途径来排毒氨。蚊子还可以使用来自谷氨酰胺的酰胺基团的氮像脊椎动物一样产生尿酸，更令人惊讶的是，蚊子通过一些鱼类和两栖动物使用的相同代谢途径将尿酸水解为尿素。由于这些多种代谢途径的协调操作可以防止血液中蚊子组织中致命的氨浓度，因此促使我们知道这些代谢途径如何相互连接和调节。因此，赠款应用的主要目的是阐明氮废物的代谢调节，并分析参与蚊子中氨代谢的C和N分子的通量。这些研究将使用RNA干扰和质谱技术的组合进行。尝试使用生物学方法来控制蚊子种群的尝试取决于对蚊子生物学和代谢的彻底理解。因此，如果我们提出的实验成功地进行了成功，它们将打开新的令人兴奋的发现，这些发现可用于设计和实施更好的蚊子控制策略。将追求以下具体目标：1-确定蚊子中氮废物调节的机制。 2-研究蚊子中参与氨代谢的C和N分子的通量。根据我们的初步数据，氮废物的代谢调节似乎在AE中受到严格调节。埃及蚊子。因此，我们的目标是了解这种调节机制如何控制成年雌性蚊子的氨代谢。