Virology Core

病毒学核心

基本信息

批准号：
8660818
负责人：
TING-TING WU
金额：
$ 11.14万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-19 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8660818
关键词：
Animals Antibodies Biological Assay Biological Response Modifiers Blood Cell Culture System Cell Line Cells Collection Complement Complex Dendritic Cells Development Electronic Mail Endothelial Cells Ensure Epithelial Cells Experimental Models Fibroblasts Firefly Luciferases Foundations Future Gene Expression Genes Genome Goals Grant Herpesviridae Herpesviridae Infections Human Human Herpesvirus 4 Human Herpesvirus 8 Immune Immune response In Vitro Individual Infection Institutes Knowledge Laboratories Latent Virus Libraries Lymphatic Endothelial Cells Lymphoma Malignant Neoplasms Measures Mission Modeling Mus Nature Open Reading Frames Oral Pathogenesis Procedures Production Program Research Project Grants Proteins Protocols documentation Reagent Recombinants Recommendation Reporter Reporter Genes Research Research Project Grants Rodent Satellite Viruses Services Suggestion System Time TimeLine Transcript Transportation Vaccines Viral Viral Genes Viral Proteins Virion Virus Virus Diseases Work base cell type cost gammaherpesvirus genetic manipulation in vitro Model in vivo in vivo Model link protein lytic replication macrophage meetings monocyte mutant novel vaccines prevent programs promoter recombinant virus research study tumor vaccine development virology

项目摘要

Human gamma-herpesviruses, including Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein Barr virus (EBV) are associated with a variety of cancers. Vaccines against EBV and KSHV would reduce the occurrence of virus-associated cancers. Major challenges to vaccine development for herpesviruses include the immunologically silent nature of latency and the large collections of immune evasion genes encoded by the viruses. Our goal is to understand the interactions between the virus and the host immune response. The knowledge will be utilized to develop new vaccine strategies. This Program will focus the research on KSHV. However, KSHV does not have robust lytic replication or a small animal infection model. To complement the studies in KSHV, this Program will also exploit an experimental model based on a closely related rodent virus, murine gammaherpesvirus 68 (MHV-68, also known as yHV-68, or MuHV-4), for proof of principle experiments. All of the research projects in this Program will investigate the effects of cellular factors on various aspects of viral replication and viral immune evasion mechanisms. Thus, it will be more efficient to organize a core that can provide a centralized, coordinated, and cost-efficient service which includes providing assays, reagents, and expertise on KSHV and MHV-68. Core B will generate and provide recombinant KSHV and MHV-68 viruses. In addition, the Core will provide in vitro cell culture systems and the virological assays with protocols, reagents and controls.

包括Kaposi的肉瘤相关疱疹病毒（KSHV）和Epstein Barr病毒（EBV）在内的人γ-疱疹病毒与多种癌症有关。针对EBV和KSHV的疫苗将减少与病毒相关的癌症的发生。疱疹病毒疫苗开发的主要挑战包括潜伏期的免疫学性质和病毒编码的大量免疫逃避基因收集。我们的目标是了解病毒与宿主免疫反应之间的相互作用。知识将用于制定新的疫苗策略。该计划将把研究重点放在KSHV上。但是，KSHV没有强大的裂解复制或小动物感染模型。为了补充KSHV的研究，该程序还将利用基于密切相关的啮齿动物病毒的实验模型Murine Gammaherpesvirus 68（MHV-68，也称为YHV-68或MUHV-4），以证明原理实验的证明。该计划中的所有研究项目都将研究细胞因素对病毒复制和病毒免疫逃避机制的各个方面的影响。因此，组织一个可以提供集中式，协调和经济高效的服务的核心将更加有效，其中包括提供KSHV和MHV-68的测定法，试剂和专业知识。核心B将产生并提供重组KSHV和MHV-68病毒。此外，核心将提供体外细胞培养系统和病毒学测定法，并提供方案，试剂和对照。