Development of Point-of-Care Diagnostics for Norovirus

诺如病毒即时诊断的开发

基本信息

批准号：
8884532
负责人：
Suri Saranathan Iyer
金额：
$ 41.57万
依托单位：
GEORGIA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-01 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8884532
关键词：
Accounting Antibodies Antigens Antiviral Agents Archives Binding Biological Assay Biosensor Blood Group Antigens Capsid Clinical Detection Development Diagnostic Discipline Disease Outbreaks Epithelial Cells Equipment Exhibits Feces Food Gastroenteritis Gastrointestinal tract structure Generations Genotype Goals Gold Health care facility Human Human Resources Infection Lateral Lead Libraries Link Mucins Nature Norovirus Patients Performance Phase Polysaccharides Positioning Attribute Prevention RNA Viruses Research Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Sampling Scientist Seasons Sensitivity and Specificity Side Solutions Structure Study Section Sulfhydryl Compounds Surface Testing Therapeutic Training Ursidae Family Vaccines Viral Virus Water analog base functional group gastrointestinal innovation nanoparticle novel novel diagnostics novel strategies pathogen point of care point-of-care diagnostics prospective rapid diagnosis user-friendly

项目摘要

Project Summary. Noroviruses, a group of nonenveloped single-stranded RNA viruses, are the leading cause of gastroenteritis outbreaks and account for 21 million cases in the US annually. Current prevention and control options are limited with no vaccines or therapeutics available. Antibody- based norovirus diagnostic kits suffer from low sensitivity and are dependent on the antibody used. Rapid, accurate, point of care diagnostic kits are critical to detect and isolate infected personnel. We propose a novel approach by using synthetic glycans as capture molecules. In this proposal, an interdisciplinary team will develop rapid, point of care diagnostics for the sensitive detection of norovirus. In Specific Aim 1, we will use a modular synthetic strategy to generate synthetic analogs of histo-blood group antigens and Lewis antigens, attachment factors for norovirus. In Specific Aim 2, we will develop and evaluate glycan based diagnostic platforms using panels of norovirus strains In Specific Aim 3, candidate diagnostic platforms will be compared side-by-side evaluated using clinical samples from both outbreaks and sporadic cases of gastrointestinal illness. Accomplishing the specific aims in this proposal is expected to yield rapid, robust, point of care, user friendly diagnostics for the precise and sensitive detection of all human norovirus strains.

项目摘要。诺如病毒是一组无包膜单链 RNA 病毒，是导致感染的主要原因美国每年有 2100 万例胃肠炎爆发。当前的由于没有可用的疫苗或治疗方法，预防和控制选择有限。抗体- 基于诺如病毒的诊断试剂盒灵敏度低且依赖于抗体用过的。快速、准确的即时诊断试剂盒对于检测和隔离感染者至关重要人员。我们提出了一种使用合成聚糖作为捕获分子的新方法。在根据这项提议，一个跨学科团队将开发快速的护理点诊断方法诺如病毒的灵敏检测。在具体目标 1 中，我们将使用模块化合成策略来生成组织血型抗原和Lewis抗原的合成类似物，附着诺如病毒的影响因素。在具体目标 2 中，我们将开发和评估基于聚糖的诊断使用诺如病毒株组的平台在特定目标 3 中，候选诊断平台将使用来自暴发和散发的临床样本进行并排比较评估胃肠道疾病的病例。预计实现本提案中的具体目标产生快速、稳健、即时、用户友好的诊断，以实现精确、灵敏的检测所有人类诺如病毒株。