Development of Point-of-Care Diagnostics for Norovirus

诺如病毒即时诊断的开发

基本信息

批准号：
8470127
负责人：
Suri Saranathan Iyer
金额：
$ 18.09万
依托单位：
GEORGIA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-01 至 2014-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8470127
关键词：
Accounting Antibodies Antigens Antiviral Agents Archives Binding Biological Assay Biosensor Blood Group Antigens Capsid Clinical Detection Development Diagnostic Discipline Disease Outbreaks Epithelial Cells Equipment Exhibits Feces Food Gastroenteritis Gastrointestinal tract structure Generations Genotype Goals Gold Health care facility Human Human Resources Infection Lateral Lead Libraries Link Mucins Nature Norovirus Patients Performance Phase Polysaccharides Positioning Attribute Prevention RNA Viruses Research Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Sampling Scientist Seasons Sensitivity and Specificity Side Solutions Structure Study Section Sulfhydryl Compounds Surface Testing Therapeutic Training United States Ursidae Family Vaccines Viral Virus Water analog base functional group gastrointestinal innovation nanoparticle novel novel diagnostics novel strategies pathogen point of care point-of-care diagnostics prospective public health relevance rapid diagnosis user-friendly

项目摘要

DESCRIPTION (provided by applicant): Noroviruses, a group of non-enveloped single-stranded RNA viruses, are the leading cause of gastroenteritis outbreaks and account for 21 million cases in the United States annually. Current prevention and control options are limited with no vaccines or therapeutics available. Antibody-based norovirus diagnostic kits suffer from low sensitivity and are dependent on the antibody used. Rapid, accurate, point-of-care diagnostic kits are critical to detect and isolate infected personnel. We propose a novel approach by using synthetic glycans as capture molecules. In this proposal, an interdisciplinary team will develop rapid, point-of-care diagnostics for the sensitive detection of norovirus. In Specific Aim 1, we will use a modular synthetic strategy to generate synthetic analogs of histo-blood group antigens and Lewis antigens, attachment factors for norovirus. In Specific Aim 2, we will develop and evaluate glycan based diagnostic platforms using panels of norovirus strains In Specific Aim 3, candidate diagnostic platforms will be compared side-by-side evaluated using clinical samples from both outbreaks and sporadic cases of gastrointestinal illness. Accomplishing the specific aims in this proposal is expected to yield rapid, robust, point of care, user friendly diagnostics for the precise and sensitive detection of all human norovirus strains.

描述（由申请人提供）：诺如病毒是一组非发达的单链RNA病毒，是胃肠炎爆发的主要原因，每年在美国占2100万例病例。当前的预防和控制选项受到限制，没有可用的疫苗或治疗剂。基于抗体的诺如病毒诊断试剂盒的灵敏度低，并且取决于所使用的抗体。快速，准确，护理点诊断套件对于检测和隔离感染人员至关重要。我们通过使用合成聚糖作为捕获分子提出了一种新的方法。在此提案中，跨学科团队将开发快速的，即保健的诊断，以敏感地检测诺如病毒。在特定目标1中，我们将使用模块化合成策略来生成诺如病毒的附着因子的组织血管抗原和刘易斯抗原的合成类似物。在特定的目标2中，我们将使用诺如病毒菌株3中的诺如病毒菌株3，将使用来自胃肠道疾病的暴发和零星病例的临床样本进行并排评估的候选诊断平台，开发和评估基于聚糖的诊断平台。预计该提案中的具体目标将产生快速，健壮，点护理，用户友好的诊断，可确切和敏感的所有诺如病毒菌株。