Vicarious Neural Activity, Genetic Differences and Social Fear Learning

替代神经活动、遗传差异和社交恐惧学习

• 批准号: 8593315
• 负责人: Jules B Panksepp
• 金额: $ 5.7万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8593315
• 关键词: Address; Adolescent; Amygdaloid structure; Applications Grants; Aptitude; Autistic Disorder; Autoradiography; Behavior; Behavioral; Biological Models; Biopsy; Brain; Brain Mapping; Brain region; Child; Conditioned Stimulus; Cues; Data; Detection; Development; Diagnosis; Diagnostic; Distress; Emotional; Empathy; Exhibits; FOS gene; Fellowship; Freezing; Fright; GABA Antagonists; Genetic; Genetic Models; Genomics; Grant; Heart Rate; Heterogeneity; Human; Immunohistochemistry; Individual; Infusion procedures; Knowledge; Laboratories; Learning; Maps; Measures; Metabolic; Modeling; Monkeys; Motor; Mouse Strains; Mus; Muscimol; Neurons; Pain; Perception; Physiologic Monitoring; Physiological; Procedures; Process; Publishing; Reporting; Research; Resolution; Rodent; Site; Social Behavior; Social Environment; Stress; Techniques; Thalamic structure; Therapeutic Intervention; Tissues; Training; Training Programs; autism spectrum disorder; base; conditioned fear; experience; fluorodeoxyglucose; genetic technology; in vivo; mirror neuron; mouse model; neurochemistry; neurophysiology; novel; programs; relating to nervous system; research study; response; social; trait; transcription factor; vocalization

Project Summary Autism spectrum disorders (ASDs) are characterized by several broad diagnostic features, with one of the most prominent being an inability for social or emotional reciprocity. The long-term objective of this grant proposal is to identify specific sites in the brain that can be used as targets for the development of more effective and more specific pharmaco-therapeutic interventions for social ailments that characterize conditions like ASD. The proposed experiments will utilize a model of autism (i.e., the BALB/cJ-C57BL/6J model of sociability) to elucidate regions of the mammalian brain that are involved in the detection and response to stress in others. The research strategy for this grant proposal is based on the premise that development of a robust model of sociability will generate fundamental knowledge regarding the mammalian ¿social brain¿ in both healthy and diseased states. The specific aims of this proposal can be summarized within the context of the training program for the PI: Specific Aim 1 - Delineate regions of the brain that express vicarious metabolic and transcriptional responses to stress, which will provide the PI with a training opportunity to acquire expertise in metabolic brain mapping and inducible transcription factor immunohistochemistry. Specific Aim 2 - Employ a localized brain inactivation procedure to modulate a behavioral response that is sensitive to the induction of stress in others, which will allow the PI to acquire expertise in procedures that are used for site-specific, reversible brain inactivation techniques. Specific Aim 3 - Characterize the physiological activity of individual neurons in response to presentation of cues that indicate stress in others, which will give the PI an excellent training experience in state-of-the-art electrophysiological recording techniques.

项目摘要 自闭症谱系障碍（ASD）的特征是几个广泛的诊断特征，其中最突出的是无法获得社交或情感互惠。该赠款提案的长期目标是确定大脑中的特定部位，可以用作开发更有效，更具体的药物治疗干预措施，以针对特征ASD等条件的社会疾病。提出的实验将利用一种自闭症模型（即BALB/CJ-C57BL/6J社交能力模型）来阐明参与其他人的哺乳动物大脑区域，这些区域涉及其他人的压力。 该赠款提案的研究策略是基于这样的前提：在健康和患病状态下，社交能力的强大模型的发展将产生有关哺乳动物的基本知识。 可以在PI培训计划的背景下总结本提案的具体目的： 具体目的1-表达对压力的替代代谢和转录反应的大脑区域，这将为PI提供训练机会，以获取代谢脑映射和诱导转录因子免疫组织化学方面的专业知识。 具体目的2-采用局部大脑灭活程序来调节对其他人诱导压力敏感的行为反应，这将使PI获得用于用于特定地点的，可逆的脑失活技术的程序方面的专业知识。 特定的目标3-表征单个神经元的体育活动，以响应表明他人压力的线索的介绍，这将使PI在最先进的电生理记录技术方面具有出色的训练经验。