ELECTROPHYSIOLOGY CORE

电生理学核心

• 批准号: 8431386
• 负责人: GLENN DILLION
• 金额: $ 15.08万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8431386
• 关键词: Age; Age-Months; Aging; Alzheimer' s Disease; Animals; Area; Behavior assessment; Blinded; Brain; Cognitive; Data; Electrophysiology (science); Ensure; Estrogens; Frequencies; Genes; Goals; Hippocampus (Brain); Homer 1; Homer 1a; Human Resources; Impaired cognition; Individual; Injury; Intervention; Laboratories; Learning; Long-Term Potentiation; Measurement; Mediating; Memory; Mitochondrial Proteins; Mus; N-Methyl-D-Aspartate Receptors; Neurons; Phosphoric Monoester Hydrolases; Process; Progesterone; Protein Isoforms; Proteins; Rattus; Regulation; Research Personnel; Seizures; Signal Transduction; Signaling Molecule; Stroke; Synaptic Transmission; Testing; Work; age related; animal age group; animal resource; base; cognitive function; cost effective; improved; motor impairment; nervous system disorder; neuropathology; neuroprotection; novel; novel therapeutic intervention; operation; organizational structure; oxidative damage; prevent; programs; research study; vasodilator-stimulated phosphoprotein

The purpose of the Electrophysiology Core is to conduct long-term potentiation recording experiments for all projects of this program. Long-term potentiation (LTP), an enhancement of synaptic transmission following high frequency stimulation, is believed to be the cellular basis of learning and memory. The mechanism of LTP has been studied widely in the hippocampal CA1 area, and many studies have demonstrated that efficacy of LTP consistently correlates with assessments of learning and memory/ cognitive function. Thus, the Electrophysiology Core will conduct LTP experiments to provide a functional assessment of cognitive function, and how it may be influenced with conditions and interventions tested in the projects. The Electrophysiology Core has the following Specific Aims: 1): Conduct hippocampal CA1 long-term potentiation recording experiments for projects 1, 2, 3 and 4. The Electrophysiology Core laboratory has established hippocampal CA1 LTP recording in mice, including those up to 24 months of age (Projects, 1, 3 and 4) and rats (Project 2). The Core will conduct all LTP experiments, analyze all data (in conjunction with the biostatistical component of the Administration Core), and provide results to the relevant Project Director. The Electrophysiology Core Director and Core personnel conducting LTP studies will be blinded to the treatment to which individual animals will have been exposed. 2): Provide an organizational structure for Project Directors to ensure a timely and cost-effective completion of required long-term potentiation studies. The Core Director will oversee overall operations of the Electrophysiology Core. As the Core will be conducting studies for four projects on various age groups of animals, the Core Director will work with Project Directors and Director of the Animal Resources and Behavioral Assessment Core (Core B) to coordinate electrophysiology studies with timings of other endpoints for each Project. The experiments to be conducted by the Electrophysiology Core will assist the Projects of this program in their attempt to discover the adverse changes that occur in the brain with aging, neurological diseases such as Alzheimer's Disease, and injuries such as stroke. Understanding these adverse changes should help us to discover new therapeutics and interventions to treat these conditions.

电生理学核心的目的是进行长期增强记录实验 对于该计划的所有项目。长时程增强 (LTP)，突触传递的增强 高频刺激后，被认为是学习和记忆的细胞基础。这 海马 CA1 区 LTP 的机制已被广泛研究，许多研究表明 证明 LTP 的功效始终与学习和记忆评估相关/ 认知功能。因此，电生理学核心将进行 LTP 实验，以提供功能性 认知功能的评估，以及它如何受到测试的条件和干预措施的影响 项目。电生理学核心有以下具体目标： 1)：进行海马 CA1 项目 1、2、3 和 4 的长期增强记录实验。电生理学核心 实验室已在小鼠（包括 24 个月大的小鼠）中建立了海马 CA1 LTP 记录 （项目 1、3 和 4）和大鼠（项目 2）。核心将进行所有 LTP 实验，分析所有数据（在 与管理核心的生物统计部分结合起来），并向相关部门提供结果 项目总监。进行 LTP 研究的电生理学核心主任和核心人员将 对个体动物所接受的治疗不知情。 2）：提供组织 项目总监的结构，以确保及时且具有成本效益地完成所需的长期任务 增强作用研究。核心主任将监督电生理学核心的整体运营。作为 核心将针对不同年龄组的动物进行四个项目的研究，核心主任将 与项目总监以及动物资源和行为评估核心（核心 B）总监合作 协调电生理学研究与每个项目其他终点的时间安排。 电生理学核心进行的实验将协助该项目 他们试图发现大脑因衰老、神经系统疾病而发生的不利变化。 阿尔茨海默病等疾病和中风等损伤。了解这些不利的变化 应该帮助我们发现新的疗法和干预措施来治疗这些疾病。