Electrophysiological Assessment

电生理评估

• 批准号: 8516547
• 负责人: Carlos T Cepeda
• 金额: $ 10.12万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8516547
• 关键词: Animal Experiments; Animal Model; Animals; Brain; Cellular biology; Collaborations; Consultations; Cultured Cells; D Cells; Data; Development; Electrophysiology (science); Faculty; Image; Individual; Instruction; Laboratories; Mental Retardation and Developmental Disabilities Research Centers; Modeling; Monitor; Mus; Mutation; Neurons; Persons; Physiological; Quality Control; Rattus; Research Personnel; Role; Schedule; Services; Slice; Time; Training; base; cellular imaging; experience; intellectual and developmental disability; meetings; research study; tissue fixing

The Core will be administered by Dr. Michael Levine. His role will be to oversee all Core services. Dr. Cepeda will provide training and instruction in electrophysiological assessment and in some cases will help perform the physiological experiments. Core services will be accessed first by consultation with Drs. Levine and Cepeda. The purpose of the consultation will be to determine which services are needed and when and how they might best be provided. Drs. Levine and Cepeda will meet with the investigator to determine the services needed and set up a time-line for provicling the service. Thus, these services are available on a firstcome, first-served basis. IDDRC investigators that need aid in performing electrophysiological analyses will have access to these Core services which are not to our knowledge presently available at UCLA except when collaborations are set up between investigators. Training and/or use of the Core set-ups will be performed according to an individualized schedule that depends on the sophistication and previous experience of the Core user. Electrophysiological experiments are labor intensive and time-consuming. It is our experience that only one investigator (or perhaps two at most depending on the services they need) will be able to use the Core at one time. We expect that most projects will require one to two months to complete (depending on availability of cultured cells and animals when slices are made), although training in electrophysiology may take longer depending on the ability of the person being trained. Once an individual is trained, they will be able to use the facility to perform experiments. The experiments and the data collected will be monitored both by Drs. Levine and Cepeda. Our experience is that weekly meetings with the investigator collecting the data are sufficient for quality control and assessment. The Physiological Assessment Core will interact directly with Core D, Cell Biology and Cellular Imaging to coordinate electrophysiological and imaging experiments on the same animals or on fixed tissue after electrophysiology is performed and Core E, Animal Models Core to determine availability of animals for experiments.

核心课程将由 Michael Levine 博士管理。他的职责是监督所有核心服务。博士。 Cepeda 将提供电生理评估方面的培训和指导，并在某些情况下帮助进行生理实验。核心服务将首先通过咨询 Drs 获得。莱文和塞佩达。咨询的目的是确定需要哪些服务以及何时以及如何最好地提供这些服务。博士。 Levine 和 Cepeda 将与调查员会面，以确定所需的服务并制定提供服务的时间表。因此，这些服务按照先到先得的原则提供。 需要帮助进行电生理分析的 IDDRC 调查人员将能够获得这些核心服务，据我们所知，加州大学洛杉矶分校目前无法提供这些核心服务，除非调查人员之间建立合作。核心设置的培训和/或使用将根据个性化时间表进行，该时间表取决于核心用户的复杂程度和以前的经验。 电生理学实验是劳动密集型且耗时的。根据我们的经验，只有一名调查员（或者最多两名，取决于他们需要的服务）能够同时使用核心。我们预计大多数项目需要一到两个月才能完成（取决于制作切片时培养细胞和动物的可用性），尽管电生理学培训可能需要更长时间，具体取决于接受培训的人员的能力。一旦个人接受培训，他们将能够使用该设施进行实验。实验和收集的数据将由 Drs 进行监控。莱文和塞佩达。我们的经验是，每周与收集数据的研究者举行会议足以进行质量控制和评估。 生理评估核心将直接与核心 D、细胞生物学和细胞成像相互作用，以协调在进行电生理学后对同一动物或固定组织进行的电生理和成像实验，并与核心 E、动物模型核心相互作用，以确定实验动物的可用性。