Application of Omics in Lung Disease

组学在肺部疾病中的应用

• 批准号: 8575263
• 负责人: Claire M Doerschuk
• 金额: $ 12.33万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8575263
• 关键词: Acute Lung Injury; Address; Area; Asses; Award; Bioinformatics; Biological Markers; Blood; Chronic Obstructive Airway Disease; Clinical Research; Computerized Medical Record; Cystic Fibrosis; DNA; Data; Databases; Doctor of Philosophy; Endothelial Cells; Epithelial Cells; Faculty; Gene Expression Profile; Genes; Genome; Genomics; Goals; Hypoxia; Individual; Knowledge; Lung; Lung diseases; Medicine; Mentored Clinical Scientist Development Program; Mentors; Mentorship; Molecular; Mucous body substance; Pneumonia; Positioning Attribute; Primary Ciliary Dyskinesias; Process; Proteome; Proteomics; Qualifying; RNA; RNA Sequences; Research; Research Personnel; Systems Analysis; Systems Biology; Technology; Training; base; biobank; career; career development; cohort; complex biological systems; design; genome wide association study; macrophage; meetings; metabolomics; microbiome; multidisciplinary; programs; skills

DESCRIPTION (provided by applicant): The long-term goal for our K12 program, "Application of Omics in Lung Disease", is to provide multidisciplinary career development that will equip MD, PhD or MD/PhD scholars with the knowledge and skills to apply pan- omics and integrative approaches to elucidating the genomic and molecular bases of lung diseases. Our Lung Omics Program will provide opportunities for two highly qualified scholars per year, who are in year 1 or 2 of their faculty positions, to understand and apply omics technologies to cutting edge questions in their developing research programs focused on the pathobiology of lung diseases and personalized medicine. The rationale is based upon the clear need for training in the use of omics. The design of our Program addresses this need by providing opportunities in forefront omics, bioinformatics and clinical research. Our Program consists of four omics, leading expertise in bioinformatics, integrative systems analysis of complex biological systems, and clinical research, as well as opportunities to use cohorts in large databases, biobanks and electronic medical record. Our omics include genomics, proteomics, metabolomics and microbiome. Genomics offers opportunities in COPD, pneumonia, acute lung injury, cystic fibrosis, primary ciliary dyskinesia, and environmental lung disease, using microarrays, RNA sequencing, GWAS to asses modifier genes, biomarkers and transcriptomes to study the DNA and RNA genomes. Proteomics focuses on the blood and airway proteomes, both the mucus proteome and the exosome proteome, and pulmonary epithelial and endothelial cells. Metabolomics includes the metabolome of the airway, both epithelial cells and macrophages, in CF and COPD, as well as the effect of hypoxia. Microbiomics pursues the airway microbiome in COPD, CF, PCD, environmental lung disease and pneumonia. This Lung Omics Career Development Program offers robust and cutting edge bioinformatics and longstanding programs in systems biology and integrative analyses of omics data. Clinical research programs are numerous and focused in these same areas of lung disease, offering our scholars opportunities and expertise in many lung diseases on which to base their omic studies. In addition to the 15 faculty mentors leading these omics and associated areas, 12 additional faculty who utilize multiple omics within their research programs also serve as mentors. Scholars will be selected through a rigorous application process. Mentorship is a critical component of our program; each scholar will have a primary and secondary mentor, as well as additional mentors as appropriate for their career development. Each scholar will have a scholarly oversight committee who meet regularly and provide guidance in career development. Each scholar will be supported for 2-3 years and will apply for individual K or R awards as appropriate. Our Program will thus facilitate the career development of our scholars and prepare them for successful careers as independent investigators using omics, bioinformatics and integrative systems analyses to pursue important questions in lung diseases.

描述（由申请人提供）：我们的 K12 项目“组学在肺部疾病中的应用”的长期目标是提供多学科职业发展，使医学博士、博士或医学博士/博士学者具备应用的知识和技能阐明肺部疾病的基因组和分子基础的全景组学和综合方法。我们的肺组学计划每年将为两名担任教职第一年或第二年的高素质学者提供机会，让他们理解组学技术并将其应用于他们正在开发的研究项目中的前沿问题，重点是肺部疾病的病理学和个性化医疗。其基本原理是基于对组学使用培训的明确需求。我们项目的设计通过提供前沿组学、生物信息学和临床研究的机会来满足这一需求。我们的计划包括四个组学、生物信息学、复杂生物系统的综合系统分析和临床研究方面的领先专业知识，以及使用大型数据库、生物库和电子病历中的队列的机会。我们的组学包括基因组学、蛋白质组学、代谢组学和微生物组学。基因组学为慢性阻塞性肺病、肺炎、急性肺损伤、囊性纤维化、原发性纤毛运动障碍和环境性肺病提供了机会，利用微阵列、RNA测序、GWAS评估修饰基因、生物标志物和转录组来研究DNA和RNA基因组。蛋白质组学重点关注血液和气道蛋白质组、粘液蛋白质组和外泌体蛋白质组，以及肺上皮细胞和内皮细胞。代谢组学包括 CF 和 COPD 中气道的代谢组，包括上皮细胞和巨噬细胞，以及缺氧的影响。微生物组学研究 COPD、CF、PCD、环境性肺病和肺炎中的气道微生物组。该肺组学职业发展计划提供强大且前沿的生物信息学以及系统生物学和组学数据综合分析方面的长期计划。临床研究项目众多，并且集中在肺部疾病的这些相同领域，为我们的学者提供了许多肺部疾病的机会和专业知识，作为他们的组学研究的基础。除了领导这些组学和相关领域的 15 名教师导师之外，另外 12 名在其研究项目中利用多种组学的教师也担任导师。学者将通过严格的申请程序进行选拔。指导是我们计划的重要组成部分；每位学者都会有一名主要和次要导师，以及适合其职业发展的额外导师。每位学者都会有一个学术监督委员会，定期开会并提供职业发展指导。每位学者将获得 2-3 年的支持，并将酌情申请个人 K 或 R 奖。因此，我们的计划将促进我们学者的职业发展，并为他们作为独立研究人员的成功职业生涯做好准备，利用组学、生物信息学和综合系统分析来探索肺部疾病的重要问题。