System for advanced automated 3D microvascular analysis in neuroplasticity

用于神经可塑性的先进自动化 3D 微血管分析系统

基本信息

批准号：
8731273
负责人：
Paul Angstman
金额：
$ 29.99万
依托单位：
MICROBRIGHTFIELD, LLC
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-06 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8731273
关键词：
Address Aging Algorithms Archives Biomedical Research Biotechnology Blood Vessels Brain Brain Diseases Caliber Collaborations Communities Complex Computer software Destinations Development Devices Encephalitis Equipment Feasibility Studies Fluorescence Future Generations Goals Grant Image Image Analysis Individual Learning Length Light Manuals Manufacturer Name Marketing Mental Health Microscope Microscopic National Institute of Mental Health Nerve Degeneration Neuronal Plasticity Neurons Neurosciences Research Phase Physiological Play Procedures Process Recovery Reporting Research Research Personnel Role Signal Transduction Slide Small Business Innovation Research Grant Societies Specimen Staining method Stains Synaptic plasticity System Techniques Technology Testing Three-Dimensional Image Time Tissue Sample Tissues Traumatic Brain Injury Trees United States National Institutes of Health Validation Variant advanced system base brain tissue density experience fighting fluorescence microscope improved innovation insight migration neurogenesis novel phase 2 study prototype public health relevance reconstruction research and development therapeutic development tool treatment strategy two-dimensional two-photon usability

项目摘要

DESCRIPTION (provided by applicant): It has become evident that the microvasculature (i.e., the vasculature visible at the light microscopic level) plays a critical role in the plasticity of he brain under various physiological and pathological conditions, including fundamental processes in neuroplasticity such as axonal, dendritic and synaptic plasticity during brain development, learning, recovery from traumatic brain injury and brain inflammation, aging and neurodegeneration, as well as during neurogenesis (i.e., the generation of new neurons) and the migration of neuronal precursors (to their final destination in the brain). Accordingly, investigators have attempted for more than 30 years to trace, reconstruct, visualize and quantitatively characterize the microscopic three-dimensional (3D) micro-angioarchitecture of the brain in normal and pathological tissue (3D microangioarchitectonics). However, there has been very little use of 3D microangioarchitectonics in neuroscience research. This is due to the paucity of tools to study 3D microangioarchitectonics. We therefore propose to create Vesselucida, an innovative software product to perform advanced, interactive and automatic 3D microangioarchitectonics in normal and pathological brain tissue. This system will allow significant advancements in studies addressing the roles of microvessels on various aspects of neuroplasticity in neuroscience research, as well as in pharmacological and biotechnology research and development as the basis for the development of innovative treatments to fight complex brain diseases. Accordingly, the development of Vesselucida represents clear progress beyond the state-of-the-art, with great benefits for the neuroscience research community and society in general.

描述（由申请人提供）：很明显，微脉管系统（即在光学显微镜水平上可见的脉管系统）在各种生理和病理条件下的大脑可塑性中发挥着关键作用，包括神经可塑性的基本过程，例如大脑发育、学习、从创伤性脑损伤和脑炎症中恢复、衰老和神经变性以及神经发生过程中的轴突、树突和突触可塑性新神经元）和神经元前体的迁移（到达大脑中的最终目的地）。因此，30多年来，研究人员一直在尝试追踪、重建、可视化和定量表征正常和病理组织中大脑的微观三维 (3D) 微血管结构（3D 微血管结构）。然而，3D 微血管构造学在神经科学研究中的应用很少。这是由于缺乏研究 3D 微血管结构的工具。因此，我们建议创建 Vesselucida，这是一种创新软件产品，可在正常和病理脑组织中执行先进、交互式和自动的 3D 微血管构建学。该系统将使研究微血管在神经科学研究中神经可塑性各个方面的作用以及药理学和生物技术研究和开发方面取得重大进展，作为开发对抗复杂脑部疾病的创新疗法的基础。因此，Vesselucida 的开发代表了超越最先进水平的明显进步，为神经科学研究界和整个社会带来了巨大的好处。