Automated 3D quantitative analysis of dendritic spines imaged with light microsco

使用光学显微镜成像的树突棘的自动 3D 定量分析

基本信息

批准号：
8643290
负责人：
Paul Angstman
金额：
$ 59.27万
依托单位：
MICROBRIGHTFIELD, LLC
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-01-12 至 2016-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8643290
关键词：
Acceleration Address Agaricales Algorithms Anatomy Autistic Disorder Automation Benchmarking Biomedical Research Brain Central Nervous System Diseases Classification Code Collaborations Communities Complex Computer software Dendrites Dendritic Spines Detection Drug abuse Elements Filopodia Goals Head Image Image Analysis Impaired cognition Information Storage Knowledge Label Laboratories Learning Light Maintenance Marketing Memory Mental disorders Microscope Microscopic Modeling Morphologic artifacts Morphology Motion National Institute of Mental Health Neuraxis Neurobiology Neurodegenerative Disorders Neurons Neurosciences Neurosciences Research New York Performance Phase Physiological Prevention Procedures Process Provider Research Research Personnel Research Support Schizophrenia Shapes Slice Slide Societies Structure Synapses TNFRSF5 gene Techniques Technology Testing Three-Dimensional Image Tissues Vertebral column Writing base improved in vivo imaging innovation interest medical schools novel portability programs prototype reconstruction software development tissue preparation tool treatment strategy validation studies virtual

项目摘要

DESCRIPTION (provided by applicant): In this Lab to Marketplace proposal, we aim to develop the MBF SpineStudio software incorporating the innovative, laboratory-based NeuronStudio software created at the Mount Sinai School of Medicine. Our SpineStudio software will enable automated detection, reconstruction and morphological classification of the structural analysis of dendritic spines. By creating this commercial software, we will improve the existing technology by providing validated, supported, and fully-documented software for automated 3D quantitative dendritic spine analysis. This software will integrate the specific advantages of the NeuronStudio software for a combination of neuron labeling, imaging (including in vivo), and image pre- processing techniques applied by the user without requiring expert knowledge in software programming. The software product will offer automated detection, reconstruction, and separate classification of complex spines (cup-shaped, multi-headed or branched, filopodia). Providing this innovative functionality will help research in important fields such as neurodegenerative diseases, learning, and memory.

描述（由申请人提供）：在本实验室到市场提案中，我们的目标是开发 MBF SpineStudio 软件，其中包含西奈山医学院创建的基于实验室的创新 NeuronStudio 软件。我们的 SpineStudio 软件将实现树突棘结构分析的自动检测、重建和形态分类。通过创建此商业软件，我们将通过提供经过验证、受支持和完整记录的软件来改进现有技术，用于自动 3D 定量树突棘分析。该软件将集成 NeuronStudio 软件的特定优势，将用户应用的神经元标记、成像（包括体内）和图像预处理技术相结合，而无需软件编程方面的专业知识。该软件产品将提供复杂脊柱（杯形、多头或分支、丝状伪足）的自动检测、重建和单独分类。提供这种创新功能将有助于神经退行性疾病、学习和记忆等重要领域的研究。