Mapping neuronal circuitry for aversion in the developing vertebrate brain

绘制脊椎动物大脑发育中厌恶的神经回路

• 批准号: 8657028
• 负责人: Su Guo
• 金额: $ 19.44万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8657028
• 关键词: Animals; Anxiety; Behavior; Behavioral Genetics; Biochemical; Brain; Breeding; Communities; Development; Disease; Enhancers; Environment; Etiology; FOS gene; Foundations; Future; Gene Transfer Techniques; Genes; Genetic; Genetic Screening; Genomics; Human; In Situ Hybridization; Knowledge; Label; Lead; Life; Light; Location; Maps; Mediating; Methods; Molecular; Neurons; Neurosciences; Outcomes Research; Pathway interactions; Pattern; Preclinical Drug Evaluation; Regulatory Element; Reporter; Reporting; Research; Rewards; Side; System; Technology; Tetanus Toxin; Therapeutic; Transgenic Animals; Transgenic Organisms; Visual; Zebrafish; addiction; avoidance behavior; base; field study; improved; in vivo; innovation; interest; motivated behavior; neural circuit; neuronal circuitry; neuronal patterning; neuropsychiatry; novel; optogenetics; public health relevance; screening

DESCRIPTION (provided by applicant): Disturbance in neuronal circuits critical for mediating reward and aversion underlies a number of neuropsychiatric disorders. However, genes and pathways that control the assembly and function of the reward and aversion neuronal circuits remain poorly defined. In this exploratory R21 application, we propose to employ larval zebrafish to study neuronal circuits underlying aversion, because the system offers great opportunity for high throughput genetic and drug screening in the context of a developing vertebrate brain. We have discovered that larval zebrafish find the dark environment aversive thereby preferring to stay in the light side. Our central hypothesis is that this dark avoidance behavior represents an excellent paradigm for understanding the development of neuronal circuitry underlying aversion at molecular and cellular levels.

描述（由申请人提供）：神经元电路中的干扰对于中介奖励和厌恶至关重要的是许多神经精神疾病的基础。但是，控制奖励和厌恶神经元电路的组装和功能的基因和途径的定义仍然很差。在此探索性R21应用中，我们建议采用幼虫斑马鱼研究厌恶厌食的神经元回路，因为该系统为在发育中的脊椎动物大脑的背景下提供了高吞吐量遗传和药物筛查的绝佳机会。我们发现，幼虫斑马鱼发现黑暗环境厌恶，从而倾向于保持在光线。我们的中心假设是，这种黑暗回避行为代表了理解分子和细胞水平上基础厌恶基础神经元电路发展的出色范式。