600 MHz NMR Instrument Upgrade at Northwestern University

西北大学 600 MHz NMR 仪器升级

• 批准号: 8247293
• 负责人: Ishwar Radhakrishnan
• 金额: $ 26.74万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247293
• 关键词: Aging; Biological; Biological Factors; Biology; Biomolecular Nuclear Magnetic Resonance; Chemicals; Chemistry; Clinical; Contrast Media; Detection; Development; Diagnostic; Electron Transport; Funding; Funding Applicant; Infectious Agent; Kinesin; Magnetic Resonance Imaging; Physiologic pulse; Regulation; Research; Research Personnel; Resolution; Resources; Shapes; Structure; Synthesis Chemistry; Time; United States National Institutes of Health; Universities; base; college; design; functional/structural genomics; inhibitor/antagonist; instrument; instrumentation; novel; programs; protein protein interaction; research study; structural biology; transcription factor; working group

DESCRIPTION (provided by applicant): Funding is requested for replacing the console of a high-resolution 600 MHz NMR spectrometer at Northwestern University's Biological NMR Center, a unique resource providing structural and chemical biologists access to high-field instrumentation within a single facility for inter- disciplinary research. Since 1990, with support from NIH and NSF, Northwestern researchers have established and maintained an outstanding resource for macromolecular NMR that has had a significant positive impact on the research programs of multiple investigators both within the college and the university at large. The 600 MHz spectrometer, which is the one and only NMR instrument in the facility, is heavily and continuously used. However, the reliability of this instrument has decreased lately because of a range of problems (including a most persistent FIFO underflow error problem) associated with an aging console, which was last replaced in 1999. The acquisition of a new console along with a replacement compressor (aka cold pack) for the cold probe would cater to the heavy demand for NMR time by a user base that comprises seven well-funded major research groups working on a variety of basic and clinical problems in biology or at the chemistry-biology interface. The research topics being studied include structure-function analyses of transcription factors, structural and functional genomics of infectious agents, electron transfer via protein-protein interactions, structural characterization of natural products, structural biology of kinesin regulation, design and development of novel inhibitors and contrast agents for magnetic resonance imaging and diagnostic applications, and novel synthetic chemistry approaches for medicinal applications. The projects have strong structural and/or functional foci and all of them will benefit from the added sensitivity (mainly from improvements in hardware that have occurred in the past decade), but most significantly, added reliability afforded by a new console and cold probe compressor. The proposed instrument will have all the capabilities to perform any contemporary biomolecular NMR pulse sequence experiment. Since the magnet and cold probe (other than the compressor) have been determined to be in good shape, there are no plans to replace either of them. The new console will have four channels with the first channel for 1H excitation, decoupling and detection, a second channel for excitation, decoupling and broadband detection of biologically-relevant heteronuclei, and a dual channel for excitation and decoupling of biologically-relevant heteronuclei, and pulsed field gradient accessories.

描述（由申请人提供）：请求资助更换西北大学生物 NMR 中心的高分辨率 600 MHz NMR 波谱仪控制台，该中心是一个独特的资源，为结构和化学生物学家提供在单一设施内使用高场仪器进行国际研究的机会。 ——学科研究。自1990年以来，在支持下 在 NIH 和 NSF 的支持下，西北大学的研究人员建立并维护了出色的大分子 NMR 资源，这对学院和整个大学内多个研究人员的研究项目产生了重大的积极影响。 600 MHz 光谱仪是该设施中唯一的核磁共振仪器，被大量且持续地使用。然而，由于与老化控制台相关的一系列问题（包括最持久的 FIFO 下溢错误问题），该仪器的可靠性最近有所下降，该控制台最后一次更换是在 1999 年。购买了新控制台以及替换压缩机冷探针的冷包（又名冷包）将满足用户群对 NMR 时间的巨大需求，该用户群由七个资金雄厚的主要研究小组组成，致力于生物学或化学-生物学界面的各种基础和临床问题。正在研究的研究主题包括转录因子的结构功能分析、感染因子的结构和功能基因组学、通过蛋白质-蛋白质相互作用的电子转移、天然产物的结构表征、驱动蛋白调节的结构生物学、新型抑制剂和对比的设计和开发用于磁共振成像和诊断应用的试剂，以及用于医学应用的新型合成化学方法。这些项目具有强大的结构和/或功能重点，所有这些项目都将受益于增加的灵敏度（主要来自过去十年中硬件的改进），但最重要的是，新的控制台和冷探针压缩机提供的可靠性增加。拟议的仪器将具备执行任何当代生物分子核磁共振脉冲序列实验的所有功能。由于磁铁和冷探头（压缩机除外）已确定状态良好，因此不打算更换其中任何一个。新控制台将有四个通道，其中第一个通道用于1H激发、解耦和检测，第二个通道用于生物相关异核的激发、解耦和宽带检测，双通道用于生物相关异核的激发和解耦，以及脉冲场梯度配件。

项目成果

A capped Tudor domain within a core subunit of the Sin3L/Rpd3L histone deacetylase complex binds to nucleic acid G-quadruplexes.

Sin3L/Rpd3L 组蛋白脱乙酰酶复合物核心亚基内的加帽 Tudor 结构域与核酸 G-四链体结合。

• 发表时间: 2022-02
• 作者: Marcum, Ryan Dale;Hsieh, Joseph;Giljen, Maksim;Justice, Emily;Daffern, Nicolas;Zhang, Yongbo;Radhakrishnan, Ishwar
• 通讯作者: Radhakrishnan, Ishwar

The neuronal transcription factor Myt1L interacts via a conserved motif with the PAH1 domain of Sin3 to recruit the Sin3L/Rpd3L histone deacetylase complex.

神经元转录因子 Myt1L 通过保守基序与 Sin3 的 PAH1 结构域相互作用，以招募 Sin3L/Rpd3L 组蛋白脱乙酰酶复合物。

• 发表时间: 2020
• 影响因子: 3.5
• 作者: Marcum, Ryan Dale;Radhakrishnan, Ishwar
• 通讯作者: Radhakrishnan, Ishwar