Xenbase: a Xenopus Model Organism Database

Xenbase：非洲爪蟾模式生物数据库

• 批准号: 8678968
• 负责人: PETER D VIZE
• 金额: $ 85.96万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2015-12-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8678968
• 关键词: African; Amino Acid Sequence; Animal Model; Biochemical; Bioinformatics; Biological; Biological Models; Cells; Communities; Complementary DNA; Data; Data Analyses; Data Sources; Databases; Development; Developmental Cell Biology; Disease; Education; Embryo; Embryology; Expressed Sequence Tags; Funding; Future; Gene Expression; Generations; Genes; Genome; Genomics; Goals; Health; Human; Human Genome; Image; Instruction; Length; Link; Literature; Manuals; Maps; Messenger RNA; Microarray Analysis; Microinjections; Mining; National Institute of Child Health and Human Development; Orthologous Gene; Pathway interactions; Peptide Sequence Determination; Phenotype; Play; Process; Proteins; Publications; RNA Splicing; Rana; Regulator Genes; Research; Research Personnel; Resources; Role; Services; System; TACSTD1 gene; Testing; Transgenic Organisms; Variant; Vertebrates; Xenopus; Xenopus laevis; comparative; data mining; egg; empowered; functional genomics; gain of function; gene function; high throughput screening; improved; in vivo; insight; member; model organisms databases; mutant; success; tool

Animal models provide powerful experimental tools to test gene function and interrogate biological pathways. Comparative functional genomics between humans and model organisms has led to a wealth of discoveries, and it is clear that this will continue to play an important role in unlocking the potential of the genome for human health. In this post genomic era highly annotated and easily accessible data are increasingly essential for researchers to integrate vast amounts of sequence, expression and functional data into a meaningful biological synthesis. The goal of this proposal is to maintain and enhance Xenbase; the Xenopus model organism database. The African frogs Xenopus has long been one of the premiere model organisms used by cell and developmental biologists, providing numerous fundamental insights. The large and abundant Xenopus embryos enable sophisticated embryology, easy manipulation of gene expression by microinjection and biochemical analyses. Together with recent advances in genome and EST sequencing, the generation of full-length cDNA sets, microarray analysis, functional genomics screens, gene knockdown using anitsense morpholino oligos, the development of rapid transgenics and the establishment of Xenbase has made Xenopus a powerful system for functional genomics. Xenbase is a comprehensive database that provides a portal to inter-related Xenopus data including genomic, mRNA and protein sequence, gene expression, function and publications, which is all highly integrated with NCBI and other model organism databases. The goal of this proposal is to maintain and expand Xenbase. Aim 1) Maintain and further curate the data in Xenbase by implementing Testpresso data mining and manual curation. Aim 2) Enhance Xenbase functionality by introducing morpholino and mutant phenotypes. Aim 3) Support new content on Xenbase gene pages including mRNA splicing microarrays, and a Xenopus WIKI. Aim 4) Continue and expand collaborative research and service efforts by further integrating Xenbase data with NCBI and other model organism data bases. This proposal will empower Xenopus research and provide the broader scientific community a means of accessing a wealth of Xenopus data.

动物模型提供了强大的实验工具来测试基因功能并询问生物学 途径。人类和模型生物之间的比较功能基因组学已导致了很多 发现，很明显，这将继续在解锁潜力方面发挥重要作用 人类健康基因组。在此帖子中，基因组时代高度注释且易于访问的数据是 对于研究人员来说，越来越重要的是整合大量序列，表达和功能数据 成为有意义的生物综合。该提案的目的是维护和增强Xenbase；这 爪蟾模型生物数据库。长期以来，非洲青蛙Xenopus一直是首映模式之一 细胞和发育生物学家使用的生物体提供了许多基本见解。大 和丰富的爪蟾胚胎可以实现复杂的胚胎学，对基因表达的轻松操纵 通过显微注射和生化分析。加上基因组和EST的最新进展 测序，全长cDNA集的产生，微阵列分析，功能基因组筛选，基因 使用Anitsense Morpholino寡素，快速转基因和机构的发展敲除 Xenbase的Xenopus成为功能基因组学的强大系统。 Xenbase是一个全面的 为相互关联的爪蟾数据提供门户的数据库，包括基因组，mRNA和蛋白 序列，基因表达，功能和出版物，它们都与NCBI和其他 模型生物数据库。该提案的目的是维护和扩展Xenbase。目标1）维护 并通过实施TestPresso数据挖掘和手动策划进一步策划XENBase中的数据。目的 2）通过引入形态和突变表型来增强Xenbase功能。目标3）支持新 Xenbase基因页面上的内容，包括mRNA剪接微阵列和Xenopus Wiki。目标4） 通过进一步将Xenbase数据与 NCBI和其他模型生物数据库。该建议将赋予Xenopus研究并提供 更广泛的科学界是访问大量Xenopus数据的手段。