Development of an adipose-derived stem cell isolation matrix

脂肪干细胞分离基质的开发

基本信息

批准号：
8702193
负责人：
Martyn Darby
金额：
$ 78.92万
依托单位：
AFFINERGY, LLC
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-15 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8702193
关键词：
Adipose tissue Adult Affect Affinity Antibodies Area Aspirate substance Autologous Binding Biocompatible Materials Biodegradation Biological Biological Assay Biomechanics Blood Vessels Bone Marrow Bone Transplantation Cell Separation Cell Therapy Cells Clinical Collagen Complex Defect Development Devices Drug Formulations Elements Engineering Evaluation Fee-for-Service Plans Fostering Fracture Frequencies Gene Expression Goals Gold Growth Factor Harvest Histology Implant Liquid substance Manuals Mesenchymal Stem Cells Modeling Mononuclear Multipotent Stem Cells Operating Rooms Operative Surgical Procedures Organ Transplantation Orthopedic Surgery procedures Orthopedics Oryctolagus cuniculus Osteogenesis Palpation Patients Peptide Synthesis Peptides Phage Display Pharmaceutical Preparations Phase Population Process Protocols documentation Publishing Reagent Rest Safety Series Speed Spinal Fusion Stem cells Sterilization Surgeon System Technology Testing Tissue Engineering Tissues United States Vertebral column Work adult stem cell biomaterial compatibility bone bone healing clinical practice cost design flexibility immunogenicity implantation improved innovation osteogenic point of care protein aminoacid sequence prototype repaired research study scaffold scale up screening self-renewal tricalcium phosphate

项目摘要

DESCRIPTION (provided by applicant): Approximately 500,000 bone-grafting procedures are performed each year in the United States. Autologous iliac crest bone graft continues to be the gold standard, because it provides essential elements for bone formation: progenitor cells, an osteoconductive matrix, and osteoinductive molecules. However, iliac crest harvest is associated with a significant number of complications and often provides an inadequate volume of graft. To overcome these limitations, multipotent Mesenchymal Stem Cells (MSCs) have been added to bone graft substitutes and explored for use in tissue engineering applications, such as spinal fusions and bone defect repairs. Bone marrow derived mesenchymal stem cells (BMSCs) are well characterized in their ability to differentiate into cells and tissues of mesodermal origin; however, their frequency in the mononuclear fraction of processed bone marrow is very low. Adipose derived stem cells (ASCs) have been proposed as an alternative to BMSCs for cell therapy because the frequency of multipotent ASCs found in the stromal vascular fraction (SVF) of processed lipoaspirate can be several hundred-fold greater than the mononuclear fraction of bone marrow aspirate (BMA), depending on the donor. Currently there are only a few basic point-of-care devices to concentrate progenitor cells, which are essential elements to foster bone healing. However, these devices are limiting because they are expensive and because they either enrich a non-specific cell population potentially diluting out desired cells or they enrich a specific cell population using antibodies and consequently have safety concerns. In this phase 2 proposal, we will develop a point-of-care autologous ASC enrichment system for bone grafting that eliminates the need for ex vivo cell expansion prior to implantation.

描述（由申请人提供）：在美国，每年大约进行500,000个骨夹程序。自体峰骨移植物仍然是黄金标准，因为它为骨形成提供了必不可少的元素：祖细胞，骨传导基质和骨诱导分子。然而，iLiac Crest收获与大量并发症有关，并且通常会提供不足的移植物。为了克服这些局限性，多能间充质干细胞（MSC）已添加到骨移植物替代物中，并探索用于组织工程应用，例如脊柱融合和骨缺陷维修。骨髓衍生的间充质干细胞（BMSC）的特征在于它们分化为中胚层的细胞和组织的能力。但是，它们在加工骨髓的单核部分中的频率非常低。已经提出了脂肪衍生的干细胞（ASC）作为BMSC进行细胞疗法的替代方法，因为在加工脂肪酸的基质血管分数（SVF）中发现的多能ASC的频率可能大于骨龙单核分数的数百倍抽吸（BMA），取决于捐助者。目前，只有少数基本的护理设备可以浓缩祖细胞，这是促进骨骼愈合的重要因素。但是，这些设备之所以限制，是因为它们很昂贵，并且因为它们要么富含潜在稀释所需细胞的非特异性细胞群，要么使用抗体富含特定的细胞群，因此存在安全问题。在此第2阶段的建议中，我们将开发出一种护理点自体ASC富集系统，用于骨移植，以消除植入前离体细胞扩张的需求。