120kV Transmission Electron Microscope for a Multi-user Microscopy Facility

适用于多用户显微镜设施的 120kV 透射电子显微镜

• 批准号: 9167780
• 负责人: JOHN S CONDEELIS
• 金额: $ 45.6万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9167780
• 关键词: 120kV; Transmission; Electron; Microscope; Multi

DESCRIPTION (provided by applicant): High resolution morphological analysis is an integral part of biological investigations under both physiological and pathological conditions. The fine structure modifications induced during cell motility or cellular secretion are captured in high resolution by transmission electron microscopy (TEM) analysis. Similarly, morphological changes observed in sub-cellular organelles (late endosomes and lysosomes) during various experimental conditions can be powerfully visualized by TEM analysis. Analysis of bacteria and fungi can provide very useful information on their morphological changes under differential experimental conditions. The Major Users of this application are requesting funding of a state of the art high resolution transmission electron microscope and an associated digital camera. The requested TEM will be part of the Analytical Imaging Facility (AIF), a multi user imaging resource, and available to all trained investigators. Currently, an aging JEOL JEM-100CX TEM installed 1982, is present in the facility, does not fulfill the needs of these investigators. The common scientific need of the major users in this application is the ability to obtain high resolution imaging of cells, organelles or pathogens under different experimental conditions. A new TEM with a high brightness LaB6 electron source and CCD camera will enable high resolution imaging and digital data recording. The transmission electron microscope described in this proposal will allow the assembled major users to analyze pathogen morphology; fine structural aspect of endosomal biogenesis and protein distribution; and granule secretion which will be otherwise precluded.

描述（由申请人提供）：高分辨率的形态分析是生理和病理条件下生物学研究不可或缺的一部分。通过透射电子显微镜（TEM）分析，在细胞运动或细胞分泌过程中引起的细胞结构修饰被高分辨率捕获。同样，可以通过TEM分析在各种实验条件下观察到在各种实验条件下观察到的形态变化（晚期内体和溶酶体）。分析细菌和真菌可以提供有关其在差异实验条件下形态变化的非常有用的信息。该应用程序的主要用户要求为最先进的高分辨率传输电子显微镜和相关的数码相机提供资金。请求的TEM将是分析成像设施（AIF）的一部分，该设施是多用户成像资源，并向所有训练有素的研究人员使用。目前，设施中存在于1982年安装的JEOL JEL JEM-100CX TEM，无法满足这些调查人员的需求。在本应用程序中，主要用户的普遍科学需求是能够在不同的实验条件下获得细胞，细胞器或病原体的高分辨率成像。具有高亮度LAB6电子源和CCD摄像头的新型TEM将实现高分辨率成像和数字数据记录。该提案中描述的透射电子显微镜将使组装的主要用户能够分析病原体形态。内体生物发生和蛋白质分布的精细结构方面；和颗粒分泌物将被排除在外。