High Speed, High Resolution Slide Scanner for Research in Translational Medicine

用于转化医学研究的高速、高分辨率幻灯片扫描仪

• 批准号: 10415257
• 负责人: JOHN S CONDEELIS
• 金额: $ 47.19万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415257
• 关键词: Biomedical Research; Carcinoma; Communities; Disease; Disseminated Malignant Neoplasm; Enhancement Technology; Environment; Funding; Gene Expression Profile; Genetic Transcription; Grant; Hepatocyte; Heterogeneity; Image; Immunocompromised Host; Interdisciplinary Study; Liver; Medicine; Microscope; Microscopy; Minor; Molecular Analysis; Neoadjuvant Therapy; Neoplasm Metastasis; Noninfiltrating Intraductal Carcinoma; Patients; Proctitis; Radiation; Reproducibility; Research; Research Personnel; Resolution; Risk Marker; Scanning; Slide; Speed; Structure; Tissues; United States National Institutes of Health; Validation; Woman; cancer cell; chemotherapy; college; equipment acquisition; fluorescence imaging; imaging facilities; instrumentation; malignant breast neoplasm; mouse model; translational medicine; tumor; tumor microenvironment; Biomedical Research; Carcinoma; Communities; Disease; Disseminated Malignant Neoplasm; Enhancement Technology; Environment; Funding; Gene Expression Profile; Genetic Transcription; Grant; Hepatocyte; Heterogeneity; Image; Immunocompromised Host; Interdisciplinary Study; Liver; Medicine; Microscope; Microscopy; Minor; Molecular Analysis; Neoadjuvant Therapy; Neoplasm Metastasis; Noninfiltrating Intraductal Carcinoma; Patients; Proctitis; Radiation; Reproducibility; Research; Research Personnel; Resolution; Risk Marker; Scanning; Slide; Speed; Structure; Tissues; United States National Institutes of Health; Validation; Woman; cancer cell; chemotherapy; college; equipment acquisition; fluorescence imaging; imaging facilities; instrumentation; malignant breast neoplasm; mouse model; translational medicine; tumor; tumor microenvironment

PROJECT SUMMARY/ABSTRACT This application is an instrumentation grant from the Analytical Imaging Facility (AIF) at the Albert Einstein College of Medicine to acquire a fully automated, high-capacity, high-resolution slide scanner that can accommodate both brightfield and fluorescence imaging. The AIF of the Albert Einstein College of Medicine supports NIH-funded investigators by giving them access to state-of-the-art microscopy technologies that enhance collaborative, multidisciplinary research. Acquisition of this instrument will have a high impact on the biomedical research at Einstein and will expand the scope of NIH-funded projects at Einstein. Several projects have been identified that will heavily utilize the scanner. These include: the study of the formation of invasion promoting structures used to systemically disseminate metastatic cancer cells in carcinoma (Condeelis), correlative Intravital-Histopathologic Imaging to dissect the tumor microenvironment (Entenberg), establishment and validation of an immunocompromised radiation proctitis mouse model (Guha), studying the prometastatic effects of neoadjuvant chemotherapy in breast cancer (Karagiannis), investigating the effect of tumor environment on metastasis (Oktay), the analysis of molecular markers of risk of subsequent invasive breast cancer in women with ductal carcinoma in situ (Rohan), and the study of hepatocyte heterogeneity with respect to gene transcription signatures and their zonal distribution within the intact liver in healthy tissue as well as diseased patient tissue (Singer).There are also additional projects of Minor Users and many users in the Einstein community are anticipated. All of the projects in this application are in need of high-capacity and high- reproducibility scanning so that analyses may be applied to larger tissues, covering hundreds of fields of view, rather than the single fields of view acquired on a standard microscope.

项目摘要/摘要 该应用是来自阿尔伯特·爱因斯坦（Albert Einstein）分析成像设施（AIF）的仪器赠款 医学院获得一个完全自动化的高容量，高分辨率的幻灯片扫描仪 可容纳Brightfield和荧光成像。阿尔伯特·爱因斯坦医学院的AIF 通过使他们获得最先进的显微镜技术来支持NIH资助的研究人员 增强合作，多学科研究。获取该工具将对 爱因斯坦的生物医学研究将扩大爱因斯坦NIH资助的项目的范围。几个项目 已经确定将大量利用扫描仪。其中包括：研究入侵的研究 促进用于系统地传播癌（Condeelis）中转移性癌细胞的结构， 剖析肿瘤微环境（Entenberg）的相关内部病理学成像，建立 并验证免疫功能低下的辐射小鼠模型（GUHA） 新辅助化疗在乳腺癌（Karagiannis）中的影响，研究了肿瘤的作用 转移的环境（oktay），分子标记的分析风险随后的侵入性乳房的风险 导管癌妇女原位（Rohan）的癌症，并研究肝细胞异质性 在健康组织中完整的肝脏中以及其内部的基因转录特征及其区域分布 患者组织（Singer）。也有未成年人和许多用户的其他项目 预计社区。本应用程序中的所有项目都需要高容量和高容量 可重复性扫描，以便将分析应用于较大的组织，涵盖数百个视野， 而不是在标准显微镜上获取的视野。