microRNA gene networks regulating responses to environment

microRNA基因网络调节对环境的反应

• 批准号: 8698074
• 负责人: Victor Ambros
• 金额: $ 64.52万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8698074
• 关键词: Animals; Biological Models; Caenorhabditis elegans; Data; Development; Developmental Process; Diabetes Mellitus; Diet; Disease; Environment; Equilibrium; Family; Feedback; Food; Gene Expression; Gene Expression Regulation; Gene Targeting; Genes; Genetic; Goals; Homologous Gene; Human; Investigation; Link; Malignant Neoplasms; Mediating; Messenger RNA; Metabolic stress; Metabolic syndrome; MicroRNAs; Modeling; Molecular Genetics; Natural Immunity; Nutritional; Organism; Pathway interactions; Physiological; Physiological Processes; Play; Process; Property; Regulator Genes; Role; Signal Transduction; Source; Stress; Time; Work; abstracting; biological adaptation to stress; design; environmental change; genome-wide; genome-wide analysis; novel; pathogen; programs; public health relevance; research study; response

DESCRIPTION (provided by applicant): Abstract The broad goal of this work is to use the C. elegans model system to understand the genetic and molecular mechanisms underlying how microRNAs mediate organismal responses to the nutitional and pathogenic properties of an animal's diet, and help confer developmental and physiological robustness against environmental change. There are more than 150 distinct microRNA genes in C. elegans, including more than 20 families of microRNAs with homologs in humans. Although it is clear that microRNAs play important roles in a wide spectrum of developmental and phsyiological processes in all animals, the functions of these microRNAs are not well understood in detail. Nor is it well understood how microRNA gene expression is regulated transcriptionally or post-transcriptionally by developmental and physiological signals. Molecular and genetic approaches will be employed to produce a comprehensive, genome-wide analysis of microRNA gene regulatory networks in C.elegans, with an emphasis on identifying microRNAs that mediate responses to the nutritional and pathogenic qualities of the animals' bacterial diet diet. In depth functional analysis will be conducted for a set of specific evolutionarily conserved microRNAs that are known to act in pathways that confer developmental and physiological robustness in the context of changing dietery conditions. .

描述（由申请人提供）： 摘要这项工作的广泛目的是使用秀丽隐杆线虫模型系统来了解microRNA如何介导动物饮食对坚果和致病性能的有机体反应的遗传和分子机制，并有助于赋予对环境变化的发育和生理鲁棒性。秀丽隐杆线虫中有150多个不同的microRNA基因，其中包括20多个具有人类同源物的microRNA家族。尽管很明显，microRNA在所有动物中都在各种发育和伪造过程中起着重要作用，但这些microRNA的功能尚未详细了解。也没有很好地理解如何通过发育和生理信号在转录或转录后调节microRNA基因表达。将采用分子和遗传学方法来对甲状腺菌的microRNA基因调节网络进行全面的，全基因组的分析，重点是鉴定介导对动物细菌饮食的营养和病原体的反应的microRNA。将对一组特定的进化保守的microRNA进行深入的功能分析，这些microRNA在改变饮食条件的情况下赋予发育和生理鲁棒性的途径。 。