Protein Chemistry Core

蛋白质化学核心

• 批准号: 8554109
• 负责人: Deborah Morrison
• 金额: $ 17.54万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8554109
• 关键词: Acetylation; Amino Acid Sequence; Amino Acids; Buffers; CCR; Charge; Communities; Computer software; Digestion; Dimensions; Extramural Activities; Gel; High Pressure Liquid Chromatography; Immunoblotting; Institutes; Intramural Research; Isoelectric Focusing; Label; Laboratories; Liquid substance; Maps; Mass Spectrum Analysis; Methods; Methylation; Modification; Molecular Weight; Peptide Mapping; Peptide Sequence Determination; Peptides; Phase; Phosphoamino Acids; Phosphorylation; Phosphorylation Site; Positioning Attribute; Post-Translational Protein Processing; Process; Protein Chemistry; Proteins; Proteomics; Radioactive; Radioactive Sequencing; Research Personnel; Resources; Sampling; Serine; Services; Site; Staining method; Stains; Techniques; Technology; Threonine; Tyrosine; United States National Institutes of Health; comparative; drug modification; interest; pressure; protein aminoacid sequence; two-dimensional

During 2011-2012, the Protein Chemistry Core provided services to investigators in the NCI-CCR as well as other NIH institutes. In addition, the Protein Chemistry Core collaborated with laboratories in the extramural community. The Core performed endoproteolytic digestions on 32-P labeled protein samples and analyzed the resulting peptides by high pressure liquid chomatography, phosphoamino acid analysis and Edman degredation, resulting in the identification of sites of protein phosphorylation. The Core also processed samples for comparative 2D-gel analysis and served as a resource to investigators regarding these techniques.

2011-2012 年间，蛋白质化学核心为 NCI-CCR 以及其他 NIH 机构的研究人员提供服务。此外，蛋白质化学核心与校外社区的实验室合作。 Core 对 32-P 标记的蛋白质样品进行内切蛋白水解消化，并通过高压液相色谱法、磷酸氨基酸分析和 Edman 降解分析所得肽，从而鉴定蛋白质磷酸化位点。 Core 还处理用于比较 2D 凝胶分析的样品，并为研究人员提供有关这些技术的资源。