Genomics

基因组学

基本信息

批准号：
8555234
负责人：
Mark D ADAMS
金额：
$ 17.26万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-14 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8555234
关键词：
Address Area Biostatistics Core Cancer Center Colon Carcinoma Comprehensive Cancer Center Core Facility DNA DNA Sequence DNA Sequencing Facility Data Data Quality Development Dideoxy Chain Termination DNA Sequencing Ensure Equipment Esophageal Adenocarcinoma Faculty Family member Formalin Funding Gene Expression Genome Genomics Genotype Goals Human Resources Individual Institutes Investments Laboratories Medical Methods Paraffin Embedding Performance Persons Pilot Projects Process Records Research Activity Research Infrastructure Research Personnel Resources Robotics Sampling Services Specimen Staging Standardization Susceptibility Gene Technical Expertise Technology Tissues United States National Institutes of Health Universities Work design flexibility genetic technology high throughput analysis instrumentation medical schools medical specialties meetings member next generation success

项目摘要

The goal of the Genomics Core is to provide comprehensive support for genomic analyses carried out in each of the Case Gl SPORE Projects. The Genomics Core will capitalize on the significant investment in common and shared infrastructure that has been created at CWRU, with support from the School of Medicine, the Case Comprehensive Cancer Center, and the Howard Hughes Medical Institute. CWRU and Cancer Center core resources that are part of this SPORE have been the subject of NIH support and are partially funded as such; thus, this SPORE will be leveraging the existing support to provide each project access to state-of-the-art genome-scale analysis expertise. The Genomics Core builds on exisfing core facilities that have a significant track record of performance and success, as well as the faculty interactions which have supported the initial work for components presented within each Project of this application. The Genomics Core will be a central resource for providing high-throughput genotyping and 'next-generation' DNA sequencing capability for two Projects. In addition, the Core will develop and implement additional methods as necessary to support all Projects, including Pilot Projects. The equipment and personnel available in the Genomics Core support research activities that require instrumentation and expertise that are not available or practical in the laboratories of the individual Project Pis, and for which centralized analyses are required in order to achieve process standardization and economies-of-scale. Specifically, the Genomics Core will perform region-specific DNA capture and high-throughput DNA sequencing and genotyping in support of the individual SPORE Projects. The Genomics Core will work closely with the Specimen and Tissue Core for access to quality assessment of materials for analysis and with the Biostatistics Core for data quality assessment and analysis.

基因组学核心的目标是为在以下领域进行的基因组分析提供全面支持：每个 Case GL SPORE 项目。基因组学核心将利用对在 CWRU 学院的支持下创建了共同和共享的基础设施医学、凯斯综合癌症中心和霍华德休斯医学研究所。 CWRU 和作为 SPORE 一部分的癌症中心核心资源一直是 NIH 支持的主题，并且部分资助；因此，这个 SPORE 将利用现有的支持来为每个项目提供获得最先进的基因组规模分析专业知识。基因组学核心建立在现有核心的基础上具有良好绩效和成功记录以及教师互动的设施它们支持了该应用程序的每个项目中呈现的组件的初始工作。这基因组学核心将成为提供高通量基因分型和“下一代”的核心资源两个项目的 DNA 测序能力。此外，核心将开发和实施额外的支持所有项目（包括试点项目）所需的方法。设备及人员基因组学核心支持需要仪器和专业知识的研究活动在各个 Pi 项目的实验室中不可用或不实用，并且集中化为了实现流程标准化和规模经济，需要进行分析。具体来说， Genomics Core 将执行区域特异性 DNA 捕获和高通量 DNA 测序，基因分型支持各个 SPORE 项目。基因组学核心将与样本和组织核心用于获取材料的质量评估以进行分析并与用于数据质量评估和分析的生物统计学核心。