Robust, defined, animal-free cell culture medium for the production of vaccines

用于生产疫苗的稳定、成分确定、无动物成分的细胞培养基

基本信息

批准号：
8393363
负责人：
Steve Clyde Pettit
金额：
$ 68.99万
依托单位：
VENTRIA BIOSCIENCE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-01 至 2014-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8393363
关键词：
Address Adult Albumins Amino Acids Animals Bioreactors Cell Culture Techniques Cell Density Cell Line Cell-Matrix Junction Cells Child Culture Media Dengue Development Drug Formulations Epithelial Cells Equilibrium Europe Excision Explosion Feasibility Studies Growth Human Industry Infection Infectious Agent Licensure Nutrient Performance Phase Plants Population Process Production Productivity Proteins Protocols documentation Recombinant Proteins Recombinants Recording of previous events Rice Risk Roller Bottle Rotavirus Infections Safety Serum Serum Albumin Serum Proteins Smallpox Source Supporting Cell System Testing Transferrin Vaccine Production Vaccines Vero Cells Viral Virus Work World Health Organization base cell growth chikungunya cost disease transmission flasks immunogenicity improved influenzavirus innovation meetings novel pandemic influenza vaccine development vaccine safety

项目摘要

DESCRIPTION (provided by applicant): The Vero cell line is used in the production of viral based vaccines. The use of cell based vaccines based on the Vero cell line is growing. The majority of the vaccines in use today are produced using cell culture medium supplemented with serum. The use of animal-derived products in vaccine culture media presents a risk for the transmission of infectious disease on a wide scale. Current commercially available serum-free vaccine media are not defined, inconsistent, and do not offer the same performance as media supplemented with serum. We propose to produce a superior performance, defined, animal-free, cell culture medium for vaccine production. Our project addresses the call by agencies for a safe alternative to animal-derived components in vaccine production. PUBLIC HEALTH RELEVANCE: Epithelial cell lines such as Vero are used in the manufacture of viral based vaccines and their use is expanding. Current commercially available animal-free vaccine production media are not defined and do not offer the same performance as media supplemented with serum. We propose the industry's first defined, high performance animal-free, cell culture medium to increase the safety and the production of vaccines.

描述（由申请人提供）：Vero 细胞系用于生产基于病毒的疫苗。基于 Vero 细胞系的细胞疫苗的使用正在增长。目前使用的大多数疫苗都是使用补充有血清的细胞培养基生产的。在疫苗培养基中使用动物源性产品存在大规模传播传染病的风险。目前市售的无血清疫苗培养基没有明确定义、不一致，并且不能提供与补充血清的培养基相同的性能。我们建议生产一种性能优越、成分明确、不含动物成分的细胞培养基，用于疫苗生产。我们的项目响应了各机构对疫苗生产中动物源性成分的安全替代品的呼吁。公共卫生相关性：Vero 等上皮细胞系用于生产病毒疫苗，其用途正在扩大。目前市售的无动物疫苗生产培养基没有明确定义，并且不提供与补充血清的培养基相同的性能。我们提出业界首个明确的、高性能的无动物细胞培养基，以提高疫苗的安全性和产量。