Robust, animal-free cell culture medium for the production of vaccines

用于生产疫苗的稳定、无动物成分的细胞培养基

基本信息

批准号：
8201046
负责人：
Steve Clyde Pettit
金额：
$ 15.88万
依托单位：
VENTRIA BIOSCIENCE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-08 至 2012-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8201046
关键词：
Address Adult Amino Acids Animals Biological Cell Culture Techniques Cell Line Cells Child Culture Media Development Disadvantaged Drug Formulations Epithelial Epithelial Cells Ethanolamines Excision FDA approved Feasibility Studies Foundations Generations Growth Growth Factor Human Industry Infectious Agent Kidney MDCK cell Marketing Methods Oncogenic Performance Phase Plants Plasma Plasma Albumin Population Prions Production Productivity Property Proteins Recombinants Recording of previous events Rice Risk Safety Serum Serum Albumin Serum Proteins Solutions Source Suspension substance Suspensions System Testing Time Trace Elements Transferrin Update Vaccine Production Vaccines Vero Cells Viral Viral Vaccines Virus Vitamins World Health Organization base cell growth continuous cell line cost cost effective density disease transmission egg improved influenza virus vaccine novel vaccine safety

项目摘要

DESCRIPTION (provided by applicant): Epithelial cell lines such as Vero and MDCK are used in the production of viral based vaccines. The use of cell based vaccines is growing. The majority of the vaccines in use today are produced in medium supplemented with serum. The use of animal-derived products in vaccine culture media presents a risk for the transmission of infectious disease on a wide scale. Current commercially available serum-free vaccine media, are not defined, inconsistent, and do not offer the same performance as media supplemented with serum. We propose to produce a superior performance, defined, animal-free, cell culture medium for vaccine production. Our project addresses the call by agencies for a safe alternative to animal-derived components in vaccine production. PUBLIC HEALTH RELEVANCE: Epithelial cell lines such as Vero and MDCK are used in the generation of viral based vaccines and their use is expanding. Current commercially available animal-free vaccine production media are not defined and do not offer the same performance as media supplemented with serum. We propose the industry's first defined, high performance animal-free, cell culture medium to increase safety and the production of vaccines.

描述（由申请人提供）：上皮细胞系如 Vero 和 MDCK 用于生产基于病毒的疫苗。基于细胞的疫苗的使用正在增长。目前使用的大多数疫苗都是在补充有血清的培养基中生产的。在疫苗培养基中使用动物源性产品存在大规模传播传染病的风险。目前市售的无血清疫苗培养基没有明确定义、不一致，并且不提供与补充血清的培养基相同的性能。我们建议生产一种性能优越、成分明确、不含动物成分的细胞培养基，用于疫苗生产。我们的项目响应了各机构对疫苗生产中动物源性成分的安全替代品的呼吁。公共卫生相关性：Vero 和 MDCK 等上皮细胞系用于生产基于病毒的疫苗，并且其用途正在扩大。目前市售的无动物疫苗生产培养基没有明确定义，并且不提供与补充血清的培养基相同的性能。我们提出业界首个明确的、高性能的非动物细胞培养基，以提高疫苗的安全性和生产。