PROJECT C

项目C

• 批准号: 8277486
• 负责人: Ronny Korstanje
• 金额: $ 30.93万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8277486
• 关键词: Alleles; Data; Diet; Epigenetic Process; Gene Expression; Genetic; Genome; Genotype; High Density Lipoprotein Cholesterol; High Density Lipoproteins; Hybrids; Inbreeding; Liver; Maps; Measures; Metabolic; Metabolic syndrome; Metabolism; Methylation; Modeling; Mus; Pattern; Phenotype; Population; Publishing; Quantitative Trait Loci; RNA Sequences; RNA Splicing; Recombinants; Spliced Genes; System; Transcript; Variant; Vitamin D; base; predictive modeling; trait

Project C: Systems Genetics of Metabolism; Ron Korstanje (Jackson) The mouse has been used as a model for many metabolic traits. Hundreds of QTL studies have been published. However, most of these studies measure single traits, even though clear correlations and interactions exist for multiple traits. In addition, these studies usually do not look at epigenetic or environmental effects. In a systems genetics approach to metabolic traits, with an emphasis on HDL cholesterol levels, we combine metabolic profiling, sequencing of RNA transcripts (RNAseq), whole-genome methylation mapping, and the mapping power of intercrossed recombinant inbred (RIX) Collaborative Cross (CC) mice to map metabolic syndrome related traits, liver (allele-specific) transcripts and splice variants and their interactions, on both a standard chow diet and a vitamin D enriched diet.

项目C：代谢系统遗传学；罗恩·科尔斯坦杰（杰克逊） 小鼠已被用作许多代谢特征的模型。已开展数百项 QTL 研究 发表。然而，大多数这些研究只测量单一特征，尽管存在明显的相关性和 多个特征存在相互作用。此外，这些研究通常不考虑表观遗传或环境 影响。在代谢特征的系统遗传学方法中，重点是高密度脂蛋白胆固醇水平，我们 结合代谢分析、RNA 转录本测序 (RNAseq)、全基因组甲基化作图、 以及交叉重组近交（RIX）协作杂交（CC）小鼠的作图能力 代谢综合征相关特征、肝脏（等位基因特异性）转录物和剪接变体及其相互作用， 标准饮食和富含维生素 D 的饮食。