TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN

PRP 寡聚物在大脑中的周转率

• 批准号: 8363794
• 负责人: STANLEY B PRUSINER
• 金额: $ 3.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363794
• 关键词: Brain; Funding; Grant; Infection; Isotope Labeling; Mass Spectrum Analysis; Measures; National Center for Research Resources; Nerve Degeneration; Neurodegenerative Disorders; Nitrogen Isotopes; Nucleic Acids; Pathology; Principal Investigator; Prion Diseases; Prions; Proteome; Research; Research Infrastructure; Resources; Source; Time; United States National Institutes of Health; cost; particle; protein aggregate; protein degradation; protein metabolism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The accumulation of protein aggregates is associated with many neurodegenerative diseases. The presence of these protein aggregates is direct evidence that normal protein metabolism is disrupted. Prions are a primary example of this effect, as they are infectious proteinaceous particles. Although strains of prions have been isolated which differ in incubation time and pathology, there is no nucleic acid component of the prion particles. Infection requires only that normal protein turnover is diverted to the formation of new aggregates. Understanding the propagation of prion disease and how these aggregates cause neurodegeneration requires that we investigate changes in protein turnover due to prion infection. Using nitrogen isotope labeling we will measure protein turnover on a proteome-wide scale to assess perturbations in global protein turnover during prion disease.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 蛋白质聚集体的积累与许多神经退行性疾病有关。 这些蛋白质聚集体的存在是正常蛋白质代谢被破坏的直接证据。 朊病毒是这种效应的主要例子，因为它们是传染性蛋白质颗粒。 尽管已经分离出不同孵育时间和病理学的朊病毒菌株，但朊病毒颗粒中不存在核酸成分。 感染只需要正常的蛋白质周转转向新聚集体的形成。 了解朊病毒疾病的传播以及这些聚集体如何导致神经变性需要我们研究朊病毒感染引起的蛋白质周转变化。 使用氮同位素标记，我们将在整个蛋白质组范围内测量蛋白质周转率，以评估朊病毒病期间全球蛋白质周转率的扰动。