PROTEOMIC STUDY OF BRASSINOSTEROID RESPONSES IN PLANTS

植物中油菜素类固醇反应的蛋白质组学研究

• 批准号: 8363849
• 负责人: ZHIYONG WANG
• 金额: $ 0.37万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363849
• 关键词: Affinity Chromatography; Biochemical; Biomass; Funding; Goals; Grant; Mass Spectrum Analysis; Methods; Molecular; Molecular Genetics; National Center for Research Resources; Plants; Principal Investigator; Production; Proteins; Proteomics; Regulation; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; plant growth/development; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our long-term goal is to identify molecular tools for increasing biomass production by understanding the molecular and biochemical mechanisms of brassinosteroid (BR) regulation of plant growth and development. Our strategy to achieve this goal is to identify BR-regulated proteins using proteomic methods, and then study their functions using molecular genetic and biochemical approaches. The specific goals of this proposal include: (1) illustrate the functions of a number of BR-regulated proteins identified by proteomic analysis; (2) identify interacting proteins of the BR-regulated protein using affinity purification and mass spectrometry; (3) identify additional BR early response proteins using prefractionation followed by 2-D DIGE and iTRAQ mass spectrometry; (4) determine the functions of a number of the newly identified proteins.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们的长期目标是通过了解油菜素类固醇 (BR) 调节植物生长和发育的分子和生化机制来确定提高生物量产量的分子工具。我们实现这一目标的策略是使用蛋白质组学方法鉴定 BR 调节蛋白，然后使用分子遗传学和生化方法研究它们的功能。该提案的具体目标包括：（1）阐明通过蛋白质组分析鉴定的一些BR调节蛋白的功能； (2)使用亲和纯化和质谱鉴定BR调节蛋白的相互作用蛋白； (3) 使用预分级，然后进行 2-D DIGE 和 iTRAQ 质谱分析，鉴定其他 BR 早期反应蛋白； (4)确定一些新鉴定的蛋白质的功能。