FUNCTIONAL STUDIES OF BRASSINOSTEROID-REGULATED PROTEINS

油菜素类固醇调节蛋白的功能研究

• 批准号: 8363848
• 负责人: ZHIYONG WANG
• 金额: $ 0.13万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363848
• 关键词: Affinity Chromatography; Arabidopsis; Binding; Blood Vessels; Cell Surface Receptors; Cell division; Cell membrane; Cells; Developmental Process; Disease Resistance; Funding; Gene Expression; Genetic; Germination; Grant; Mass Spectrum Analysis; Molecular; Molecular Genetics; National Center for Research Resources; Nuclear; Phosphotransferases; Physiological Processes; Plants; Play; Principal Investigator; Productivity; Proteins; Proteomics; Research; Research Infrastructure; Resources; Role; Seeds; Signal Pathway; Signal Transduction; Source; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; cost; gene function; improved; receptor; steroid hormone; stress tolerance; success; tool; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Brassinosteroids (BRs) are plant steroid hormones, which play important roles in a wide range of physiological and developmental processes, including cell expansion, cell division, vascular differentiation, seed germination, photomorphogenesis, stress tolerance and disease resistance. Molecular genetic studies have identified several BR signal transduction components of the BR signaling pathway, through which BRs bind the cell surface receptors to regulate nuclear gene expression. However, there are still gaps in the current BR signaling pathway. In particular, the substrates of the receptor kinases that transduce the BR signal from plasma membrane to cytoplasmic components remain unknown. Despite the success of genetic studies, many genes' function cannot be discovered by forward genetic approaches, largely due to genetic redundancy. Therefore, alternative approaches are required to significantly advance our understanding of the BR signaling pathway. Thus, we have performed proteomic studies and identified BR-regulated proteins in Arabidopsis using two-dimensional difference gel electrophoresis (2-D DIGE) followed by mass spectrometry. This study aims at characterizing plasma membrane-localized BR-responsive proteins, including the BR-signaling kinases (BSKs). Functional studies of these proteins have demonstrated their key role as BRI1 substrate in the BR signaling pathway. We plan to identify additional interacting proteins of BSKs using affinity purification followed by mass spectrometry analysis. Such analysis will advance our understanding of BR signal transduction and provide molecular tools for improving plant productivity.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 油菜素类固醇（BRs）是植物类固醇激素，在多种生理和发育过程中发挥重要作用，包括细胞扩张、细胞分裂、维管分化、种子萌发、光形态发生、胁迫耐受和抗病性。分子遗传学研究已经确定了BR信号通路的几个BR信号转导元件，BR通过这些元件结合细胞表面受体来调节核基因表达。然而，目前的BR信号通路仍存在空白。特别是，将 BR 信号从质膜转导至细胞质成分的受体激酶的底物仍然未知。尽管遗传学研究取得了成功，但许多基因的功能无法通过正向遗传学方法发现，这很大程度上是由于遗传冗余。因此，需要替代方法来显着增进我们对 BR 信号通路的理解。因此，我们进行了蛋白质组学研究，并使用二维差异凝胶电泳 (2-D DIGE) 和质谱分析鉴定了拟南芥中 BR 调节的蛋白质。本研究旨在表征质膜定位的 BR 响应蛋白，包括 BR 信号激酶 (BSK)。 这些蛋白质的功能研究证明了它们作为 BR 信号通路中 BRI1 底物的关键作用。我们计划使用亲和纯化和质谱分析来鉴定 BSK 的其他相互作用蛋白。此类分析将增进我们对 BR 信号转导的理解，并为提高植物生产力提供分子工具。