SYNTHESIS OF SMALL MOLECULES TO PROBE ENZYMATIC FUNCTION

合成小分子来探测酶功能

• 批准号: 8363795
• 负责人: Danica Galonic Fujimori
• 金额: $ 1.75万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363795
• 关键词: Chemicals; Enzymes; Funding; Grant; Histones; Mass Spectrum Analysis; Measures; Methylation; Modification; Molecular Weight; National Center for Research Resources; Nucleic Acids; Nucleosomes; Pattern; Principal Investigator; Regulation; Research; Research Infrastructure; Resources; Ribosomal RNA; Role; Site; Source; Transcriptional Regulation; Translations; United States National Institutes of Health; cost; interest; macromolecule; protein phosphatase inhibitor-2; small molecule; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our lab in interested in methyl marks as modulators of protein and nucleic acid function. In the context of nucleosomes, we are interested in activity, regulation and chemical inhibition of histone demethylases, key enzymes involved in regulation of transcription. Additionally, we are investigating role of methylation of ribosomal RNA in translation. For success of these projects, it is crucial to accurately measure molecular weight of substrate macromolecules, and identify, via analysis of fragmentation patterns, exact sites of modification. Thus, these projects can not be accomplished without key inputs from mass spectrometry.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们的实验室对甲基标记作为蛋白质和核酸功能的调节剂感兴趣。在核小体的背景下，我们对组蛋白去甲基酶的活性、调节和化学抑制感兴趣，组蛋白去甲基酶是参与转录调节的关键酶。此外，我们正在研究核糖体 RNA 甲基化在翻译中的作用。为了这些项目的成功，准确测量底物大分子的分子量，并通过分析碎片模式来识别精确的修饰位点至关重要。因此，如果没有质谱的关键输入，这些项目就无法完成。