MULTI-DIMENSIONAL MICROSCOPY

多维显微镜

• 批准号: 8362644
• 负责人: Eric Olaf Potma
• 金额: $ 2.33万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362644
• 关键词: Biological; Biotechnology; Cells; Cellular Structures; Collagen; Development; Elastin; Filament; Flavins; Fluorescence; Funding; Generations; Grant; Image; Imaging Techniques; Label; Lasers; Lipids; Maps; Methods; Microscope; Microscopic; Microscopy; Morphology; National Center for Research Resources; Optical Coherence Tomography; Optics; Penetration; Phase; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Source; Specimen; Structure; System; Techniques; Tissues; United States National Institutes of Health; cost; imaging modality; in vivo; optical imaging; second harmonic; tool; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent developments in optical imaging have revolutionized the way in which we can examine cells and tissue in vivo. The number of modern optical imaging technique is continuously expanding, each offering a unique contrast mechanism and sensitivity to important bio-compounds in the system under study. For instance, tissue morphology can be non-invasively examined with optical coherence tomography (OCT). Over the past decade, the development of nonlinear methods such as two photon excited fluorescence (TPEF), second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy has given the biomedical researcher convenient tools for selectively visualizing endogenous structures without the need of labeling. The multi-dimensional approach to microscopy integrates various imaging techniques and contrast mechanisms to better assess the biological specimen. Combinations of the TPEF, SHG, CARS and OCT techniques have previously been realized into optical microscopes, however, a full integration of these imaging modalities has not been accomplished. In this proposal we push the envelope of multi-dimensional imaging in the following way: a)Combining the TPEF, SHG, CARS and OCT into a single imaging platform. b)Optimizing the image contrast and penetration depth by controlling the spectral phase. The proposed multi-dimensional imaging platform uniquely permits the three-dimensional mapping of key tissue and cell components such as flavin metabolites, elastin networks, collagen filaments and lipid pools along with the detailed tissue morphology. Such an optical assessment of the biological specimen would provide one of the most detailed microscopic views of the sample currently possible.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 光学成像的最新发展彻底改变了我们体内检查细胞和组织的方式。现代光学成像技术的数量正在不断扩大，每种技术都为所研究系统中的重要生物化合物提供了独特的对比机制和敏感性。例如，可以使用光学相干断层扫描（OCT）对组织形态进行非侵入性检查。在过去的十年中，双光子激发荧光 (TPEF)、二次谐波发生 (SHG) 和相干反斯托克斯拉曼散射 (CARS) 显微镜等非线性方法的发展为生物医学研究人员提供了方便的工具，可以选择性地可视化内源结构，而无需标签的需要。多维显微镜方法集成了各种成像技术和对比机制，以更好地评估生物样本。 TPEF、SHG、CARS 和 OCT 技术的组合先前已在光学显微镜中实现，但是，这些成像模式的完全集成尚未实现。在这个提案中，我们通过以下方式推动多维成像的发展： a) 将 TPEF、SHG、CARS 和 OCT 组合到一个成像平台中。 b)通过控制光谱相位来优化图像对比度和穿透深度。 所提出的多维成像平台独特地允许对关键组织和细胞成分（例如黄素代谢物、弹性蛋白网络、胶原丝和脂质池）以及详细的组织形态进行三维映射。对生物样本的这种光学评估将提供目前可能的最详细的样本显微视图之一。