REDOX IMAGING OF HUMAN LYMPHOMA

人类淋巴瘤的氧化还原成像

• 批准号: 8362023
• 负责人: LIN LI
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362023
• 关键词: Apoptosis; Cell Culture Techniques; Cell physiology; Flavoproteins; Fluorescence; Funding; Grant; Human; Image; Lymphoma; Magnetic Resonance; Measures; Metabolism; Mitochondria; Mus; NADH; National Center for Research Resources; Oxidation-Reduction; Principal Investigator; Research; Research Infrastructure; Resources; Role; Scanning; Signal Transduction; Source; Tissues; United States National Institutes of Health; Xenograft procedure; base; cancer therapy; chemotherapy; cost; in vivo; malignant breast neoplasm; melanoma; novel strategies; optical imaging; response; tumor progression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Redox scanning can measure the in vivo mitochondrial redox states of tissues on the basis of the fluorescence signals of NADH and Fp (oxidized flavoproteins), and Fp redox ratios (Fp/(NADH+Fp)) was shown by previous studies to predict the aggressiveness of human melanoma and breast cancer in mouse xenografts. Redox ratio is also sensitive to cellular processes including apoptosis, differentiation and metabolism. In this project we employ redox scanning to study human lymphoma cell culture and mouse xenografts with and without chemotherapy. We hope to understand more about the role of mitochondrial redox state in tumor progression and response to treatment. We may also accumulate evidence for targeting mitochondrial redox state as a novel approach of cancer therapy.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 氧化还原扫描可以根据NADH和Fp（氧化黄素蛋白）的荧光信号来测量组织体内线粒体氧化还原状态，之前的研究显示Fp氧化还原比（Fp/（NADH+Fp））可以预测攻击性小鼠异种移植物中的人类黑色素瘤和乳腺癌。氧化还原比对细胞过程也很敏感，包括细胞凋亡、分化和代谢。在这个项目中，我们采用氧化还原扫描来研究人类淋巴瘤细胞培养物和经过或未经化疗的小鼠异种移植物。我们希望更多地了解线粒体氧化还原状态在肿瘤进展和治疗反应中的作用。我们还可能积累针对线粒体氧化还原状态作为癌症治疗新方法的证据。