Animal and Pathology Core

动物和病理学核心

基本信息

批准号：
8743014
负责人：
ILYA GUKOVSKY
金额：
$ 26.65万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8743014
关键词：
Acinar Cell Adaptor Signaling Protein Address Animal Model Animal Welfare Animals Bile Acids Cell model Cell secretion Choline Disease Ensure Ethionine Evaluation Fibrosis Formalin Generations Genetic Genetic Models Germany Goals Histologic Human Individual Infusion procedures Knockout Mice Maintenance Modeling Modification Molecular Chaperones Monitor Mouse Strains Mus Necrosis Organelles Pancreas Pancreatic Diseases Pancreatic duct Pancreatitis Paraffin Embedding Pathologist Pathology Play Proteins Quality Control Reproducibility Reproducibility of Results Research Research Personnel Scientist Signaling Protein Site Site-Directed Mutagenesis Specimen System TPD52 gene Tissue Banking Tissue Banks Tissue Sample Tissues Transgenic Mice Universities abstracting acute pancreatitis clinical material clinically relevant cost cost effective dietary supplements experience gastrointestinal human disease human tissue mouse model overexpression programs success

项目摘要

Animal and Pathology Core: Summary/Abstract The Animal and Pathology Core is comprised of 2 sub-Cores. The Animal sub-Core will generate two severe experimental pancreatitis models: i) induced by choline deficient, ethionine-supplemented diet (CDE), and ii) bile-acid (taurolithocholate) pancreatic duct infusion on both wild type and Project-relevant genetically modified mice. Another critical task of this sub-Core is to generate and maintain colonies of knockout and transgenic mice used to address the shared studies as well as topics specific to individual Projects. The Pathology sub-Core will provide each Project with human tissue specimens to validate the relevance of the results obtained in animal and cell models of pancreatitis. The Pathology sub-Core will be also responsible for evaluation of all human tissue histologic sections. Due to potential variability in the quality of human tissues caused by necrosis and fibrosis, a standardized system of human tissue evaluation and quality control will be central to the Program's success. The Pathology sub-Core will also oversee the review and scoring of histopathological changes in experimental mouse models of pancreatitis as well as genetic models. The goals of the Core will be achieved by carrying out the following Specific Aims: Aim 1: To provide standardized animal models of pancreatitis, to maintain and generate colonies of genetically modified mice. 1a. Animal models of pancreatitis 1b. Maintenance of genetically modified mouse colonies 1c. Generation of new genetically modified mouse strains Aim 2: To provide human pancreatic tissues and pathohistologic evaluation of human and mouse pancreas tissues. The cost-reduction will be achieved by sharing among the Projects tissue samples from wild-type and genetically modified mice (both with and without pancreatitis) as well as human tissue specimens.

动物和病理学核心：总结/摘要动物和病理学核心由 2 个子核心组成。动物子核心将产生两种严重的实验性胰腺炎模型：i) 由缺乏胆碱、补充乙硫氨酸的饮食 (CDE) 诱导，以及 ii) 对野生型和项目相关基因的胆汁酸（牛磺石胆酸盐）胰管输注改良小鼠。该子核心的另一个关键任务是生成和维持敲除和转基因小鼠用于解决共享研究以及个别项目的特定主题。病理学子核心将为每个项目提供人体组织标本，以验证其相关性在胰腺炎的动物和细胞模型中获得的结果。病理学子核心也将负责用于评估所有人体组织组织学切片。由于人类素质的潜在差异坏死和纤维化引起的组织，人体组织评估和质量控制的标准化体系将是该计划成功的核心。病理学子核心还将监督病理学的审查和评分胰腺炎实验小鼠模型以及遗传模型的组织病理学变化。核心目标将通过实现以下具体目标来实现：目标1：提供标准化的胰腺炎动物模型，维持和产生遗传性的克隆改良小鼠。 1a.胰腺炎动物模型 1b.转基因小鼠群体的维持 1c.新的转基因小鼠品系的产生目标2：提供人类胰腺组织以及人类和小鼠胰腺的病理组织学评估组织。成本的降低将通过在项目之间共享来自野生型和转基因小鼠（患有和不患有胰腺炎）以及人体组织标本。