Functional Analysis of Sleep Promoting Neurons in Health and Disease

睡眠促进神经元在健康和疾病中的功能分析

• 批准号: 8713270
• 负责人: PAUL J SHAW
• 金额: $ 28.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8713270
• 关键词: Adult; Adverse effects; Aging; Alzheimer' s Disease; Animal Model; Animals; Attenuated; Behavior; Biological; Brain; Calcium; Camping; Cells; Chronic; Circadian Rhythms; Cognitive; Cognitive deficits; Cyclic AMP; Data; Degenerative Disorder; Disease; Dorsal; Drosophila genus; Exposure to; Frequencies; Gene Mutation; Genetic; Health; Image; Impaired cognition; Invertebrates; Life; Link; Longevity; Measures; Modeling; Molecular; Morbidity - disease rate; Neurodegenerative Disorders; Neurons; Neuropeptides; Parkinson Disease; Pathology; Pathway interactions; Patients; Pattern; Pharmaceutical Preparations; Play; Presynaptic Terminals; Process; Property; Proteins; Role; Shapes; Signal Transduction; Sleep; Sleep Deprivation; Sleep Disorders; Social Environment; Starvation; Testing; Therapeutic; Time; Vulnerable Populations; cellular imaging; fly; human disease; improved; in vivo; meetings; mortality; mutant; presenilin; research study; response; sensor; tool

DESCRIPTION (provided by applicant): We have identified a group of ~20 neurons that can be activated on-demand using Drosophila genetics. The sleep observed during activation of these neurons meets the historical definition for identifying sleep. Our data highlight the importance of these neurons for regulating sleep. In this proposal we will use live-cell imaging and in vivo electrophysiological recording from Drosophila brains to define the properties of these neurons in the intact brain. The ability to use Drosophila genetics to induce sleep provides a unique opportunity to examine whether sleep can be used as a therapeutic for slowing or attenuating cognitive impairments associated with degenerative diseases. Thus, we will determine whether inducing sleep in Drosophila models of Parkinson's and Alzheimer's disease can offset deficits in cognitive behavior.

描述（由申请人提供）：我们已经确定了一组约20个神经元，可以使用果蝇遗传学激活点播。这些神经元激活期间观察到的睡眠符合识别睡眠的历史定义。我们的数据突出了这些神经元对调节睡眠的重要性。在此提案中，我们将使用果蝇大脑的活细胞成像和体内电生理记录来定义这些神经元在完整大脑中的特性。使用果蝇遗传学来诱导睡眠的能力为检查睡眠是否可以用作减慢或衰减与退化性疾病相关的认知障碍的治疗性。因此，我们将确定帕金森氏果蝇模型和阿尔茨海默氏病诱导睡眠是否可以抵消认知行为的缺陷。