Nasal progenitor cells and olfactory neurogenesis

鼻祖细胞和嗅觉神经发生

• 批准号: 8766365
• 负责人: Bradley J Goldstein
• 金额: $ 22.12万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-17 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8766365
• 关键词: Address; Adult; Affect; Afferent Neurons; Aging; Aging-Related Process; American; Anosmia; Appointment; Area; Basal Cell; Biometry; Biophysics; Cell Therapy; Cell physiology; Cells; Clinical Treatment; Clinical Trials; Coculture Techniques; Commit; Craniocerebral Trauma; Cre-LoxP; Data; Development; Diphtheria Toxin; Disease; Elderly; Embryo; Epithelial; Ethics; Failure; Fostering; Future; Genetic; Goals; Hares; Impairment; In Vitro; Injury; Institutes; Intake; Lamina Propria; Lead; Lesion; Life; Maintenance; Maps; Mentors; Mesenchymal; Mesenchymal Stem Cells; Monitor; Mus; National Institute on Deafness and Other Communication Disorders; Natural regeneration; Neuroepithelial; Neurons; Nose; Nutritional; Olfactory Mucosa; Otolaryngology; Patients; Peripheral Blood Stem Cell; Physicians; Physiology; Population; Positioning Attribute; Process; Property; Proto-Oncogene Protein c-kit; Quality of life; Receptor Protein-Tyrosine Kinases; Regenerative Medicine; Research; Research Design; Research Personnel; Role; Safety; Scientist; Signal Pathway; Sinusitis; Smell Perception; Stem cells; System; Taste Perception; Techniques; Testing; Tissues; Training Programs; United States; Universities; Upper Respiratory Infections; Visit; Work; base; career development; clinical practice; design; effective therapy; experience; improved; in vivo; interest; meetings; neuroepithelium; neurogenesis; neuron loss; neuronal replacement; novel; professor; progenitor; programs; public health relevance; receptor; reconstitution; repaired; research study; stem; therapeutic target

DESCRIPTION (provided by candidate): The goal of this application is to provide a supervised training program that will promote the candidate's development into an independent physician - scientist. This will be accomplished through a coordinated effort in the Department of Otolaryngology and the Interdisciplinary Stem Cell Institute (ISCI), at University of Miami. This proposal will enable the candidate to pursue his interest in mechanisms of regeneration in the olfactory system through a mentored plan to study nasal stem cells and specific signaling pathways. Research Program: Although olfactory neurons in the nose are replaced continually from progenitor or stem cells, certain conditions can lead to a loss of olfactory function that likly is a consequence of failures in this repair process. Anosmia can occur following head injury, prior upper respiratory infections, active sinusitis, or as a consequence of the aging process. There are currently no effective treatments for sensorineural anosmia. The broad, long term goals of this proposal are to develop novel treatments for anosmia. Our interest in determining mechanisms regulating olfactory progenitors has led to the novel finding that olfactory cells expressing the c-kit receptor can give rise to olfactory neurons. The central hypothesis of this proposal is that c-kit (+) progenitor cells serve a key role in adult olfactory maintenance. The experiments proposed here will directly define the role of nasal c-kit (+) progenitors in olfactory neurogenesis and regeneration under various conditions. Defining the function of specific nasal stem and progenitor cells underlying olfactory neuronal replacement, and determining their regulatory mechanisms, are essential steps towards the establishment of new treatments for anosmia. Training Program: The candidate will participate in a didactic program to enhance his career development. Courses and seminars at University of Miami relating to ethics, research design, and biostatistics will be audited. Also, he will participate in intellectual activities in he Otolaryngology Department and the Stem Cell Institute. Frequent meetings with advisors will be utilized to monitor progress. Dr. Josh Hare, ISCI Director, and Dr. Nirupa Chaudhari, Professor of Physiology and Biophysics, will serve as Mentors.

描述（由候选人提供）：本申请的目的是提供监督的培训计划，将候选人的发展促进候选人的发展为独立的医生 - 科学家。这将通过在迈阿密大学的耳鼻喉科和跨学科干细胞研究所（ISCI）的协调努力来实现。该提案将使候选人能够通过研究鼻干细胞和特定信号通路的指导计划来追求对嗅觉系统再生机制的兴趣。研究计划：尽管鼻子中的嗅觉神经元不断地从祖细胞或干细胞中取代，但某些条件可能导致嗅觉功能的丧失，这可能是该修复过程中失败的结果。头部受伤，先前的上呼吸道感染，活性鼻窦炎或衰老过程后可能发生厌食。目前没有有效的感觉神经性厌食症的治疗方法。该提议的长期目标是为厌食症开发新的治疗方法。我们对确定调节嗅觉祖细胞的机制的兴趣导致了新发现，即表达C-KIT受体的嗅觉细胞会引起嗅觉神经元。该提议的中心假设是C-KIT（+）祖细胞在成人嗅觉维持中起关键作用。此处提出的实验将直接定义鼻C-kit（+）祖细胞在嗅觉中的作用 在各种条件下的神经发生和再生。定义特定的鼻茎和祖细胞的功能以及确定其调节机制的作用，是朝着建立新治疗厌食症的基本步骤。培训计划：候选人将参加一项教学计划，以增强他的职业发展。迈阿密大学的课程和研讨会与道德，研究设计和生物统计学有关。此外，他还将参加HE HEORORARYNGOLOGY部门和干细胞研究所的智力活动。与顾问的频繁会议将用于监控进度。 ISCI主管Josh Hare博士和生理学与生物物理学教授Nirupa Chaudhari博士将担任导师。