UCSF DVS CyTOF Mass Cytometer

UCSF DVS CyTOF 质谱流式细胞仪

基本信息

批准号：
8639996
负责人：
LEWIS Lee LANIER
金额：
$ 60万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2016-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The University of California, San Francisco (UCSF) is requesting funds for the purchase of a CyTOF C5 Mass Cytometer by DVS Sciences with Cytobank advanced data management and analytical software. Mass cytometry is a transformational new technology unavailable at UCSF that permits the identification and investigation of rare, diseased cells. The CyTOF mass cytometer marries the resolution, specificity, and sensitivity of atomic mass spectrometry to the high-throughput, single-cell analytical advantages currently provided by flow cytometry. Using stable isotopically pure (non-radioactive) metal tags, as many as forty parameters per single cell can be identified and quantified simultaneously, yielding unique biomarker signatures that distinguish a more refined analysis of cell populations. DVS Sciences is the only mass cytometer manufacturer. The impetus for this application is UCSF's missing mass cytometer capability. There are 43 scientific projects by 21 investigators from 12 different UCSF departments providing justification for funding. Acquisition of the CyTOF mass cytometer will create a shared resource with a projected usage over 2500 hours per year, demonstrating a real need for this technology. Four investigators are currently using the technology through inefficient long-distance collaborations. Clinical biopsies and human samples represent over 80% of the estimated usage of this system, potential biohazards not easily accommodated at other institutions. In response to this technology need, UCSF in a $2M renovation project is building a designated BSL2 (biosafety level) room for a mass cytometry resource. This resource will be part of an existing core facility and includes the ability to construct novel metal-tagged probes, manage CyTOF instrument operation, employ sophisticated software tools for managing and analyzing multi-parametric mass cytometer data, offer continued training and support to the user base, and educational courses to UCSF students. Institutional support will total 17% of the final cost for the CyTOF M5 Mass Cytometer.

描述（由申请人提供）：加利福尼亚大学旧金山大学（UCSF）要求使用DVS Sciences购买Cytobank Advance Advanced Data Management和Analytical Software的DVS Sciences购买Cytof C5质量细胞量。质量细胞仪是UCSF上无法使用的一种变革性的新技术，可以识别和研究罕见的患病细胞。细胞质量细胞图仪与原子质谱法的分辨率，特异性和敏感性与流式细胞术当前提供的高通量单细胞分析优势。使用稳定的同位素纯（非放射性）金属标签，可以同时识别和量化每个单个单元的40个参数，从而产生独特的生物标志物特征，从而区分了对细胞群体的更精致分析。 DVS科学是唯一的质量细胞仪制造商。该应用程序的动力是UCSF缺失的质谱仪能力。来自12个不同UCSF部门的21个研究人员提供了43个科学项目，提供了资金的理由。获取质量细胞图仪的获取将创建共享资源，每年预计使用2500小时的使用情况，这表明对这项技术的真正需求。目前，四名研究人员正在通过效率低下的长距离合作使用该技术。临床活检和人类样本占该系统估计使用的80％以上，潜在的生物危害不容易适应其他机构。为了应对这项技术需求，UCSF在一个耗资200万美元的翻新项目中正在为大规模细胞仪资源建造指定的BSL2（生物安全水平）房间。该资源将成为现有核心设施的一部分，包括构建新型金属标签探针，管理仪器操作，采用复杂的软件工具来管理和分析多参数质量细胞仪数据，为用户提供持续培训和支持的能力基础和向UCSF学生的教育课程。机构支持将总计M5质量细胞仪的最终成本总成本的17％。