OMX Blaze Structured-Illumination Microscope for a Core Facility

适用于核心设施的 OMX Blaze 结构照明显微镜

• 批准号: 8640326
• 负责人: SCOT CHARLES KUO
• 金额: $ 60万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-16 至 2016-02-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640326
• 关键词: Address; Cell Nucleus; Cell physiology; Cells; Color; Confocal Microscopy; Core Facility; Data; Distant; Equipment; Fluorescence; Funding; Image; Imagery; Label; Lateral; Life; Lighting; Microscope; Organelles; Performance; Proteins; Resolution; Specificity; Speed; Structure; System; Technology; Testing; Tissues; United States National Institutes of Health; Vendor; instrument; macromolecular assembly

DESCRIPTION (provided by applicant): As part of an established core facility, we request an OMX Blaze structured-illumination microscope (SIM) from Applied Precision. Key cellular processes occur in structures and assemblies smaller than resolvable by confocal microscopy, but still requiring the ease and specificity of fluorescence labeling. Addressing multiple experimental systems, our super-resolution needs have five constraints. (1) The volumes we must explore are often deep within cells and tissues, significantly distant from the coverslip. (2) All studies require axial super-resolution, as well as lateral super-resolution. (3) The relevant structures, spanning large protein assemblies to organelles to the nucleus, are typically macromolecular assemblies 100 nm or larger. (4) The assemblies often interact with many other molecules, requiring a robust palette of colors for simultaneous visualization. (5) For some live-cell dynamics, speed of imaging is important. After extensive testing of multiple technologies, only SIM is appropriate for our needs. Furthermore, we have preliminary SIM data for all Users, thoroughly exploring each vendor's implementation of SIM. Compared to existing equipment anywhere at Johns Hopkins, the proposed instrument will provide an order-of-magnitude superior performance that will enable key NIH-funded projects.

描述（由申请人提供）：作为已建立的核心设施的一部分，我们从应用精确度请求OMX BLAZE结构化显微镜（SIM）。关键的细胞过程发生在与共聚焦显微镜可分解的结构和组件中发生，但仍需要荧光标记的易于和特异性。解决多个实验系统时，我们的超分辨率需求有五个限制。 （1）我们必须探索的体积通常在细胞和组织内，与盖玻片显着远。 （2） 所有研究都需要轴向超分辨率以及横向超分辨率。 （3）相关结构跨越大蛋白质组件到细胞核的细胞器，通常是100 nm或更大的大分子组件。 （4）组件经常与许多其他分子相互作用，需要强大的颜色调色板才能同时可视化。 （5）对于某些活细胞动力学，成像速度很重要。在对多种技术进行了广泛的测试之后，只有SIM适合我们的需求。此外，我们为所有用户提供了初步的SIM数据，并彻底探索了每个供应商的SIMS实现。与约翰·霍普金斯（Johns Hopkins）的任何地方的现有设备相比，拟议的工具将提供较高的效果效果，以实现NIH资助的关键项目。