The Monogamous Prairie Vole: A Model for the Study of Social Behavior and Pain

一夫一妻制的草原田鼠：研究社会行为和疼痛的模型

基本信息

批准号：
8823884
负责人：
THOMAS JEFFREY MARTIN
金额：
$ 23.13万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-30 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8823884
关键词：
Absence of pain sensation Address Affect Affective Animal Model Animals Behavior Behavioral Brain Stem Breeding Clinic Clinical Research Communities Dopamine Evaluation Family Female Future Genetic Genome Grooming Hamsters Health Housing Human Hypersensitivity Injury Knock-out Laboratories Laboratory Animal Models Libraries Life Ligation Literature Maintenance Measures Mechanics Microtus Midbrain structure Modeling Mus Neurobiology Operative Surgical Procedures Oxytocin Pain Pain Research Pair Bond Partner in relationship Patients Rattus Recovery Research Research Personnel Resolution Rodent Role Running Severities Sexual Partners Site Social Behavior Social Interaction Stimulus Study models Time Treatment outcome Vasopressins Vertebrates Withdrawal behavior influence behavior measurement chronic neuropathic pain chronic pain design and construction experience forest improved male multisensory mutant nerve injury neuroinflammation nonhuman primate novel offspring painful neuropathy partial recovery prairie vole pre-clinical preference productivity loss psychosocial public health relevance response sciatic nerve sex sham surgery social social attachment tool

项目摘要

DESCRIPTION (provided by applicant): The objectives of this proposal are to develop the monogamous prairie vole as a novel laboratory animal model for preclinical pain research. Two specific aims are proposed: (1) determine the response of prairie voles to nerve injury using both reflexive withdrawal measures of hypersensitivity and non-reflexive measures of the affective/motivational components of pain, and (2) determine how pair-bonding between prairie voles or disruption of pair bonds in these animals affects pain responses and resolution of pain after nerve injury. Prairie voles are unique among most vertebrate species in that, like humans, they form lifelong monogamous pair bonds with both the male and female undertaking significant roles in raising offspring. Clinically, patients with chronic neuropathic pain that reprt stable and supportive relationships with a partner are shown to have improved treatment outcomes than those without such social enrichment. The mechanisms by which positive social interaction may mitigate pain have not been explored in a preclinical setting, in part due to the lack of similar social interactions between humans and typical laboratory animal models such as mice and rats, or even non-human primates. The prairie vole has been utilized for two decades to understand the neurobiology of pair bond formation and social interaction, and the prominent role of oxytocin, vasopressin, and dopamine in these behaviors are now appreciated. The prairie vole genome has been sequenced and BAC libraries are currently being constructed for design of knockouts or genetic mutants and this species will continue to be developed as an important model species. This provides the pain research community with an opportunity to explore ways in which this species can be utilized to address pain neurobiology, including the influence of human-like social behaviors, which no other species can presently afford. These studies will develop the prairie vole as an important tool for preclinical pain research, as well a advance the understanding of how pain influences social behavior in these animals for the prairie vole research community at large.

描述（由申请人提供）：该提案的目的是开发一夫一妻制的草原Vole，作为临床前疼痛研究的新型实验室动物模型。提出了两个具体的目的：（1）通过反射性戒断措施对疼痛的情感/动机组成部分的反射性戒断措施来确定草原对神经损伤的反应，以及（2）确定在这些动物中，Prairie竞争对这些动物的疼痛反应和疼痛损伤的伴侣键之间的偶数互相损害和疼痛的损害。草原田鼠在大多数脊椎动物物种中都是独一无二的，因为它们像人类一样，形成终生的一夫一妻对键，男性和女性都在提高后代中发挥了重要作用。在临床上，与没有这种社交富集的患者相比，与伴侣的慢性神经性疼痛患者相比，与伴侣的稳定和支持性关系的患者相比，其治疗结果得到了改善。积极的社会互动可能减轻疼痛的机制尚未在临床前环境中探索，部分原因是人类与典型的实验室动物模型（如小鼠和大鼠，甚至是非人类灵长类动物）之间缺乏类似的社交互动。现在已经利用了大草原的Vole来了解成对键形成和社会相互作用的神经生物学，以及催产素，加压素和多巴胺在这些行为中的重要作用。草原vole基因组已被测序，目前正在为敲除或遗传突变体设计BAC库，该物种将继续作为重要的模型物种开发。这为疼痛研究界提供了一个机会，可以探索该物种可以利用该物种来解决疼痛神经生物学的方式，包括类似人类的社会行为的影响，而这些社会行为目前无法负担。这些研究将开发草原vole作为临床前疼痛研究的重要工具，也可以提高人们对疼痛如何影响这些动物的社会行为的理解。