Recombinant Virus and Vector Core

重组病毒和载体核心

• 批准号: 8541141
• 负责人: YAN YUAN
• 金额: $ 27万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-08 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8541141
• 关键词: Arts; B-Lymphocytes; Cells; Complementary DNA; Consultations; Databases; Engineering; Event; Generations; Genetic Recombination; Goals; Grant; Herpesviridae; Human Herpesvirus 8; Infection; Instruction; Laboratories; Lentivirus; Methods; Molecular; Mutagenesis; Mutation; Online Systems; Personal Communication; Production; Reagent; Recombinants; Resources; Role; Services; Site; Suggestion; Viral Genome; Viral Proteins; Viral Vector; Virion; Virus; base; design; expression vector; large scale production; mutant; particle; programs; recombinant virus; repository; response; small hairpin RNA; vector

SUMMARY (See instructions): The Recombineering and Virus Production core will be responsible for 1) recombinant virus design consultation, 2) vector and viral reagent repository; 3) generation of site-directed mutations in Bacmid KSHV, and 4) large scale production of recombinant and wild-type KSHV. These goals will be accomplished by a centralized laboratory, providing a set of standardized services, including state ofthe art methods in herpes virus, molecular engineering and recombination-based mutagenesis (i.e. Kan-SacB, Kan-rpsL, Gal-K, and LoxP-kan for conditional knock-outs), production of recombinant Lenti-virus expression vectors for cDNA and shRNA, and production of KSHV from transfected 293 cells (recombinant viruses), GFP tagged viruses with specific, mutations, or BCBL1 cells (wild-type virus). Since it is expected that this program project will generate unique resources for research, the core will be responsible for storage of recombinant viruses, bacmids, reagents for recombineering and Lenti-virus production, as well as management of adequate databases that users can access and search via a web-based browser. Expertise in all aspects of protein and virus production will be provided to the projects for the lifetime of grant support.

摘要（请参阅说明）：重组和病毒生产核心将负责1）重组病毒设计咨询，2）载体和病毒试剂储存库； 3）在BACMID KSHV中产生定向突变，4）重组和野生型KSHV的大规模产生。 These goals will be accomplished by a centralized laboratory, providing a set of standardized services, including state ofthe art methods in herpes virus, molecular engineering and recombination-based mutagenesis (i.e. Kan-SacB, Kan-rpsL, Gal-K, and LoxP-kan for conditional knock-outs), production of recombinant Lenti-virus expression vectors for cDNA and shRNA, and production of KSHV from transfected 293个细胞（重组病毒），GFP标记具有特异性，突变或BCBL1细胞（野生型病毒）的病毒。由于预计该计划项目将生成独特的研究资源，因此核心将负责存储重组病毒，BACMID，重组和Lenti-Virus生产的试剂，以及对用户可以通过基于Web的浏览器访问和搜索的充分数据库的管理。在授予支持的一生中，将向项目提供有关蛋白质和病毒生产各个方面的专业知识。