Elucidating Essential Components of Bone Marrow Metastasis

阐明骨髓转移的重要组成部分

• 批准号: 8903811
• 负责人: Jungwoo Lee
• 金额: $ 24.15万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-10 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8903811
• 关键词: 3-Dimensional; Adipocytes; Adipose tissue; Biocompatible Materials; Blood Circulation; Blood Vessels; Bone Marrow; Bone Marrow Neoplasms; Bone Marrow Stem Cell; Breast; Cancer Biology; Cell Culture Techniques; Cells; Cessation of life; Development; Discontinuous Capillary; Distant; Engineering; Engraftment; Goals; Growth; Growth and Development function; Hematopoietic stem cells; Human; Image; Implant; In Vitro; Location; Lung; Malignant Neoplasms; Mammary Neoplasms; Marrow; Mediating; Mesenchymal Stem Cells; Metastatic Neoplasm to the Bone Marrow; Microfluidics; Modeling; Molecular; Mus; Neoplasm Metastasis; Osteoblasts; Pharmaceutical Preparations; Positioning Attribute; Preparation; Process; Prostate; Prostatic Neoplasms; Public Health; Recruitment Activity; Research; Research Personnel; Simulate; Site; Skin; Stromal Cells; System; Technology; Therapeutic; Tissue Engineering; Tissue Model; Training; Tumor Biology; Undifferentiated; Universities; abstracting; analog; bone; cancer cell; carcinogenesis; chemokine; improved; in vivo; leukemia; malignant breast neoplasm; microchip; microsystems; migration; neoplastic cell; oncology; osteogenic; prevent; prostate cancer cell; scaffold; therapeutic target; tumor; tumor growth; tumor microenvironment

Project Summary/Abstract Metastasis to the bone marrow is observed in a number of fatal cancers. Tumor cells preferentially migrate to the bone cavity as a function of composition of the bone marrow. We have developed a tissue engineered analogue of the bone marrow and plan to use this construct to identify the key stromal cellular components of a bone marrow metastatic niche. We propose to: 1) Develop a microfluidic platform to capture migratory tumor cells in vitro, 2) Create tumor-stroma microenvironments on-chip, and 3) In vivo comparison and treatment study of BMSC scaffolds on tumor growth. This project will provide essential training to candidate in microfluidics, imaging, and cancer biology during the K99 training period and propel him to be a successful independent investigator in the field of biomaterials and oncology as an R00 investigator at a research university.

项目概要/摘要 在许多致命癌症中观察到骨髓转移。肿瘤细胞优先迁移至 骨腔作为骨髓成分的函数。我们开发了一种组织工程 骨髓的类似物，并计划使用该结构来识别骨髓的关键基质细胞成分 骨髓转移微环境。我们建议：1）开发微流控平台来捕获迁移性肿瘤 体外细胞，2) 在芯片上创建肿瘤基质微环境，以及 3) 体内比较和治疗 BMSC支架对肿瘤生长的影响研究。该项目将为候选人提供必要的培训 在 K99 培训期间，他学习了微流控、成像和癌症生物学，并推动他成为一名成功的 生物材料和肿瘤学领域的独立调查员，作为 R00 调查员在一项研究中 大学。